DEVELOPMENT AND DIFFERENTIATION OF AIRWAY EPITHELIUM

Project: Research project

Description

Identification of specific markers for airway cells is an important
prerequisite in studies of the development and differentiation of pulmonary
airway cells. We plan to identify non-serum proteins in lung lavage and
raise monoclonal antibodies to the proteins. The antibodies will be tested
for their reactivity to and specificity for airway cells by
immunocytochemistry. Availability of markers specific of different airway
cells will facilitate the study of development of the cells. Cell specific
markers will be used to study the effect of a number of factors on the
development of airway cells, using morphology, immunocytochemistry,
morphometry and quantitation of proteins as the methologic approaches. Studies of the regulation of development of airway epithelium may be
important in the treatment of patients with bronchopulmonary dysplasia.
Identification and structural analyses of the cell specific proteins is
also likely to provide insight into the function of different airway
cells. Knowledge of the function of specific proteins of airway cells may
provide clues to the pathogenesis of chronic obstructive pulmonary disease
and emphysema. We will isolate cell specific proteins, and plan to examine
their amino acid sequence and, in the long run, isolate the genes for the
proteins. Availability of cDNA probes for the genes will be valuable in
studies of gene regulation of the airway cell specific proteins.
StatusFinished
Effective start/end date9/30/869/29/90

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Epithelium
Proteins
Genes
Bronchopulmonary Dysplasia
Pulmonary Emphysema
Bronchoalveolar Lavage
Cell Differentiation
Amino Acid Sequence
Complementary DNA
Immunohistochemistry
Monoclonal Antibodies
Antibodies

ASJC

  • Medicine(all)