NEW APPROACHES TO DNA MAPPING--ELECTRONIC IMAGING

Project: Research project

Project Details

Description

Separation and analysis of restriction fragments by gel
electrophoresis and Southern blotting are vital to the mapping of
chromosomal DNA. At present, however, the information obtained
from gels and blots is severely limited by the nonlinear response,
limited dynamic range, low sensitivity and cumbersome processing
inherent in the photographic methods used to record the
distribution of fluorophore-stained or radionuclide-labeled DNA.
This project will exploit recent advances in electronic imaging
and computer technology that make possible accurate and rapid
quantitation of the mass distribution of fluorophore-stained
restriction fragments on gels and radionuclide-labeled probes on
Southern blots. Computer programs for the storage and analysis of
images of gels and blots will be developed to increase
significantly the quality and quantity of information obtained
while reducing the time required for data collection and
processing.
StatusNot started