Project: Research project

Project Details


DESCRIPTION: (Adapted from the Investigator's Abstract): SMVT is a tranporter
which, when expressed in mammalian cells, induces the transport of
pantothenate, biotin, and lipoate. Since SMVT mediates the uptake of biotin
both in the intestine and the placenta, the investigator hypothesizes that the
expression of SMVT in the placenta is also subject to regulation by plasma
concentrations of biotin, pantothenate and lipoate. This will be tested in
vitro and in vivo using BeWo choriocarcinoma cells and the pregnant rat as
model systems. Studies will include analysis of changes in the transport
activity, transporter density, and the steady-state levels of the transporter
mRNA induced by changes in the concentrations of pantothenate, biotin, and
lipoate in the culture medium or maternal plasma. Immunolocalization studies
will be performed to examine the expression of the cloned transporter in the
brush border and/or basal membrane of the syncytiotrophoblast to understand the
role of SMVT in the entry and/or exit of the vitamins in the placenta. The
ontogenic development of the transporter in placenta and fetus will be studied
using rats as an animal model. A detailed functional characterization of two
vitamin C transporters, SVCTI and SVCT2, is proposed to determine features of
SVCT2 function that render the specific expression of this transporter in the
placenta more advantageous for the transplacental transfer of vitamin C.
Proposed studies will also investigate the regulation of SVCT2 expression in
rat placenta and BeWo cells in response to substrate availability and ontogenic
development of SVCT2 in rat placenta.
Effective start/end date9/1/006/30/05


  • Medicine(all)


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