Project: Research project

Project Details


A system to produce, store and analyze images of the distribution of
radiolabeled DNA, RNA and proteins in electrophoretic gets, Southern-,
northern-, western-, and dot-blots and thin-layer chromatograms will be
acquired. The same imaging system will also record x-ray diffraction
patterns that are used to determine the three dimensional structures of
proteins and complexes of proteins with DNA and RNA. The detectors in this system will be photostimulable phosphor screens that
form a latent image when exposed to beta- and x-rays. The latent image is
converted into a digital image by a laser scanner. Photostimulable
phosphor detection provides significant increases in radiometric
sensitivity, accuracy of quantitation and dynamics range compared to
conventional x-ray film. Phosphor screens have better spatial resolution,
can acquire larger images, and permit more flexible collection of image
data compared to other technologies that have been developed to replace x-
ray film. The image data system consists of three major components. A computer
controlled laser scanner will transcribe the latent image in a phosphor
screen to a digital form. The digital image will be transferred to the
disk of a multi-user file server, which will provide convenient short-term
access and cost-effective long-term storage of images files. Images will
be transferred from the server to analysis workstations that will be
provided by the Major Users of the system. Eight research groups, consisting of thirty four scientists, will be the
Major Users of the system. Each Major User Group has either active or
pending support from NIH. All are located in the Biology Department of
Brookhaven National Laboratory. The system will also be available to seven
additional research groups (a total of 25 scientists) from the Biology
Department and to scientists from the Chemistry and Medical Departments at
Brookhaven, the graduate and medical schools of the State University of New
York at Stony Brook and the Plum Island Animal Disease Center.
StatusNot started


  • National Institutes of Health: $117,000.00


Explore the research topics touched on by this project. These labels are generated based on the underlying awards/grants. Together they form a unique fingerprint.