TY - JOUR
T1 - α6β1 integrin is a potential clinical marker for evaluating sperm quality in men
AU - Reddy, Venkata Rami K.
AU - Rajeev, Shrimali K.
AU - Gupta, Vijayalakshmi
N1 - Funding Information:
Supported by the Department of Biotechnology, Government of India (grant BT/PRO587/Med/09/115/97).
PY - 2003/6/1
Y1 - 2003/6/1
N2 - Objective: To determine the clinical significance of α6β1 integrin in human spermatozoa. Design: Prospective case-controlled study. Setting: Hospital infertility clinic in India. Patient(s): Sixty-two men undergoing evaluation for infertility and 20 healthy, proven-fertile controls were selected. Intervention(s): Role of α6β1 integrin in sperm quality evaluation was determined using semen analysis, cell ELISA, flow cytometry, and in vitro sperm-egg binding. Main Outcome Measure(s): Determination of sperm-associated α6β1 integrin may help in assessing the quality of the human spermatozoa. Result(s): Expression of α6β1 integrin was significantly low in the spermatozoa obtained from subfertile men. Only 35%-40% of spermatozoa from fertile men showed a positive reaction to α6β1 antibody. The samples with high rate of acrosomal reaction showed a good expression of α6β1 and maximum binding to oocyte in an in vitro binding assay. Conclusion(s): α6β1 Integrin may be used as a clinical marker to evaluate sperm quality.
AB - Objective: To determine the clinical significance of α6β1 integrin in human spermatozoa. Design: Prospective case-controlled study. Setting: Hospital infertility clinic in India. Patient(s): Sixty-two men undergoing evaluation for infertility and 20 healthy, proven-fertile controls were selected. Intervention(s): Role of α6β1 integrin in sperm quality evaluation was determined using semen analysis, cell ELISA, flow cytometry, and in vitro sperm-egg binding. Main Outcome Measure(s): Determination of sperm-associated α6β1 integrin may help in assessing the quality of the human spermatozoa. Result(s): Expression of α6β1 integrin was significantly low in the spermatozoa obtained from subfertile men. Only 35%-40% of spermatozoa from fertile men showed a positive reaction to α6β1 antibody. The samples with high rate of acrosomal reaction showed a good expression of α6β1 and maximum binding to oocyte in an in vitro binding assay. Conclusion(s): α6β1 Integrin may be used as a clinical marker to evaluate sperm quality.
KW - Infertility
KW - Integrins
KW - Sperm-egg interaction
KW - Spermatozoa
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U2 - 10.1016/S0015-0282(03)00368-6
DO - 10.1016/S0015-0282(03)00368-6
M3 - Article
C2 - 12801565
AN - SCOPUS:0038511271
SN - 0015-0282
VL - 79
SP - 1590
EP - 1596
JO - Fertility and sterility
JF - Fertility and sterility
IS - SUPPL. 3
ER -
