β-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn

Bin Zhang, Shiwen Luo, Xian Ping Dong, Xian Zhang, Chunming Liu, Zhenge Luo, Wen Cheng Xiong, Lin Mei

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Agrin is believed to be a factor used by motoneurons to direct acetylcholine receptor (AChR) clustering at the neuromuscular junction. However, exactly how agrin mediates this effect remains unclear. Here we demonstrate that the β-catenin interacts with rapsyn, a molecule key for AChR clustering. Agrin stimulation increases the association of β-catenin with surface AChRs. Suppression of β-catenin expression inhibited agrin-induced AChR clustering, suggesting a necessary role of β-catenin in this event. The β-catenin action did not appear to require the function of T-cell factors (TCFs), suggesting a mechanism independent of TCF-mediated transcription. In contrast, prevention of β-catenin from interacting with α-catenin attenuated agrin-induced AChR clustering. These results suggest that β-catenin may serve as a link between AChRs and α-catenin- associated cytoskeleton, revealing a novel function of β-catenin in synaptogenesis.

Original languageEnglish (US)
Pages (from-to)3968-3973
Number of pages6
JournalJournal of Neuroscience
Volume27
Issue number15
DOIs
StatePublished - Apr 11 2007

Fingerprint

Catenins
Cholinergic Receptors
Cell Communication
Muscle Cells
Cluster Analysis
Agrin
TCF Transcription Factors
peripheral membrane protein 43K
Neuromuscular Junction
Motor Neurons
Cytoskeleton

Keywords

  • AChR
  • Cluster
  • Interaction
  • Rapsyn
  • α-catenin
  • β-catenin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

β-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn. / Zhang, Bin; Luo, Shiwen; Dong, Xian Ping; Zhang, Xian; Liu, Chunming; Luo, Zhenge; Xiong, Wen Cheng; Mei, Lin.

In: Journal of Neuroscience, Vol. 27, No. 15, 11.04.2007, p. 3968-3973.

Research output: Contribution to journalArticle

Zhang, B, Luo, S, Dong, XP, Zhang, X, Liu, C, Luo, Z, Xiong, WC & Mei, L 2007, 'β-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn', Journal of Neuroscience, vol. 27, no. 15, pp. 3968-3973. https://doi.org/10.1523/JNEUROSCI.4691-06.2007
Zhang, Bin ; Luo, Shiwen ; Dong, Xian Ping ; Zhang, Xian ; Liu, Chunming ; Luo, Zhenge ; Xiong, Wen Cheng ; Mei, Lin. / β-catenin regulates acetylcholine receptor clustering in muscle cells through interaction with rapsyn. In: Journal of Neuroscience. 2007 ; Vol. 27, No. 15. pp. 3968-3973.
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AU - Xiong, Wen Cheng

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