β-glucan of Ganoderma lucidum (W.Curt.:Fr.) Lloyd (aphyllophoromycetideae) cooperatively induces tumor necrosis factor-α and interleukin-6 with lipopolysaccharide by binding to dectin-1

Su Kyoung Sung, Mi Jung Kim, Dong Hee Lee, Se Young Choung, Byong Kak Kim, Ha Won Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

β-Glucan is a glucose polymer that has linkage of β-(1,3), -(1,4) and -(1,6). Exclusively found in fungal and bacterial, not in animal, cell walls, β-glucans are recognized by the innate immune system. Dendritic cells (DC) or macrophages possess pattern recognition molecules (PRM) for binding β-glucan in the pathogen-associated molecular pattern (PAMP). Recently, β-glucan receptor was cloned from DC and named dectin-1, which belongs to the type II C-type lectin family. Human dectin-1 consists of 7 exons and 6 introns. The polypeptide of dectin-1 has 247 amino acids and has cytoplasmic, transmembrane, stalk, and carbohydrate recognition domains. Dectin-1 can recognize a variety of β-1,3 and/or β-1,6 glucan linkages, but not α-glucans. In our macrophage cell line culture system, dectin-1 mRNA was detected in RAW264.7 cells by a reverse transcription/ polymerase chain reaction (RT-PCR). Dectin-1 was also detected in the murine organs of spleen, thymus, lung, and intestine. Treatment of RAW264.7 cells with β-glucans of Ganoderma lucidum (GLG) resulted in increased expression of IL-6 and TNF-α in the presence of LPS. However, GLG alone did not increase IL-6 or TNF-α. These results suggest that receptor dectin-1 cooperates with CD14 to activate signal transduction, which is very critical in immunoresponses.

Original languageEnglish (US)
Pages (from-to)193-200
Number of pages8
JournalInternational Journal of Medicinal Mushrooms
Volume7
Issue number1-2
DOIs
StatePublished - Jul 4 2005
Externally publishedYes

Fingerprint

Reishi
Glucans
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha
Dendritic Cells
Macrophages
C-Type Lectins
dectin 1
Introns
Cell Wall
Thymus Gland
Reverse Transcription
Intestines
Exons
Immune System
Signal Transduction
Spleen
Cell Culture Techniques
Carbohydrates

Keywords

  • Dectin-1
  • Ganoderma lucidum
  • IL-6
  • LPS
  • Macrophage
  • RT-PCR
  • TNF-α
  • β-glucan

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

Cite this

β-glucan of Ganoderma lucidum (W.Curt.:Fr.) Lloyd (aphyllophoromycetideae) cooperatively induces tumor necrosis factor-α and interleukin-6 with lipopolysaccharide by binding to dectin-1. / Sung, Su Kyoung; Kim, Mi Jung; Lee, Dong Hee; Choung, Se Young; Kim, Byong Kak; Kim, Ha Won.

In: International Journal of Medicinal Mushrooms, Vol. 7, No. 1-2, 04.07.2005, p. 193-200.

Research output: Contribution to journalArticle

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abstract = "β-Glucan is a glucose polymer that has linkage of β-(1,3), -(1,4) and -(1,6). Exclusively found in fungal and bacterial, not in animal, cell walls, β-glucans are recognized by the innate immune system. Dendritic cells (DC) or macrophages possess pattern recognition molecules (PRM) for binding β-glucan in the pathogen-associated molecular pattern (PAMP). Recently, β-glucan receptor was cloned from DC and named dectin-1, which belongs to the type II C-type lectin family. Human dectin-1 consists of 7 exons and 6 introns. The polypeptide of dectin-1 has 247 amino acids and has cytoplasmic, transmembrane, stalk, and carbohydrate recognition domains. Dectin-1 can recognize a variety of β-1,3 and/or β-1,6 glucan linkages, but not α-glucans. In our macrophage cell line culture system, dectin-1 mRNA was detected in RAW264.7 cells by a reverse transcription/ polymerase chain reaction (RT-PCR). Dectin-1 was also detected in the murine organs of spleen, thymus, lung, and intestine. Treatment of RAW264.7 cells with β-glucans of Ganoderma lucidum (GLG) resulted in increased expression of IL-6 and TNF-α in the presence of LPS. However, GLG alone did not increase IL-6 or TNF-α. These results suggest that receptor dectin-1 cooperates with CD14 to activate signal transduction, which is very critical in immunoresponses.",
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