2-Deoxy-d-glucose inhibition of herpes simplex virus type-1 receptor expression

J. G. Mohanty, Ken S. Rosenthal

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Growth of HEp-2 cells in 2-deoxy-d-glucose (2-DOG) supplemented media decreased the cells' binding capacity for herpes simplex virus type-1 KOS (HSV-1) but not vesicular stomatitis virus. HEp-2 cells tolerated up to 30 mM 2-DOG, but 2-DOG was toxic for Vero cells over 2 mM. The reduction in binding was maintained for at least 24 h even after careful removal of the inhibitor and growth in normal media. Complete regeneration of the receptor sites on HEp-2 cells was observed 8 h after mild trypsinization of cells grown in either normal or the 2-DOG supplemented media. Specific glycoprotein characteristics of the HSV-1 binding site were indicated by its inactivation upon trypsinization (0.1 mg per 5 × 105 cells for 30 s) and blocking by wheat germ agglutinin but not limulin. These results suggest that 2-DOG inhibits the proper expression of cell surface glycoprotein HSV-1 receptor sites on HEp-2 cells.

Original languageEnglish (US)
Pages (from-to)137-149
Number of pages13
JournalAntiviral Research
Volume6
Issue number3
DOIs
StatePublished - May 1986
Externally publishedYes

Keywords

  • 2-deoxy-d-glucose
  • HEp-2 cells
  • HSV-1
  • receptor expression

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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