Abstract
Growth of HEp-2 cells in 2-deoxy-d-glucose (2-DOG) supplemented media decreased the cells' binding capacity for herpes simplex virus type-1 KOS (HSV-1) but not vesicular stomatitis virus. HEp-2 cells tolerated up to 30 mM 2-DOG, but 2-DOG was toxic for Vero cells over 2 mM. The reduction in binding was maintained for at least 24 h even after careful removal of the inhibitor and growth in normal media. Complete regeneration of the receptor sites on HEp-2 cells was observed 8 h after mild trypsinization of cells grown in either normal or the 2-DOG supplemented media. Specific glycoprotein characteristics of the HSV-1 binding site were indicated by its inactivation upon trypsinization (0.1 mg per 5 × 105 cells for 30 s) and blocking by wheat germ agglutinin but not limulin. These results suggest that 2-DOG inhibits the proper expression of cell surface glycoprotein HSV-1 receptor sites on HEp-2 cells.
Original language | English (US) |
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Pages (from-to) | 137-149 |
Number of pages | 13 |
Journal | Antiviral Research |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - May 1986 |
Externally published | Yes |
Keywords
- 2-deoxy-d-glucose
- HEp-2 cells
- HSV-1
- receptor expression
ASJC Scopus subject areas
- Pharmacology
- Virology