TY - JOUR
T1 - A calfluxin-related peptide is present in the bag cells and atrial gland of Aplysia
AU - de Jong-Brink, Marijke
AU - Nagle, Gregg T.
AU - Dictus, Wim J.A.G.
AU - Painter, Sherry D.
AU - Broers-Vendrig, Tineke
AU - Blankenship, James E.
N1 - Funding Information:
This investigation was supported by National Science Foundation Grants BNS 85 17575 (G.T.N.) and BBS 87 11368, by National Institutes of Health Research Grants NS 22079 (S.D.P.) and NS 23169 (J.E.B.) and by Biomedical Research Support Grant RR 97925 from the UTMB Graduate School of Bio- medical Science (G.T.N.). The Florence and Marie Hall Endowment for Programs of Excellence in Education in the Biomedical Sciences provided financial support for computer facilities. We thank Dr. R. Given for assistance.
PY - 1990/7
Y1 - 1990/7
N2 - The caudodorsal cells of Lymnaea and the bag cells of Aplysia are neuroendocrine cells whose peptide products have homologous functions, i.e., regulation of egg deposition. One of the Lymnaea products, calfluxin, increases cytosolic and hence mitochondrial calcium concentrations in secretory cells of the albumen gland, an exocrine organ that secretes perivitellin fluid around the egg cells during packaging; the changes can be visualized at the ultrastructural level for quantification with the pyroantimonate precipitation technique and are correlated with changes in the secretory and biosynthetic activity in the gland. Comparable studies have now been carried out with Aplysia and indicate that the bag cells of A. californica and A. brasiliana also contain a factor with calfluxin-related activity, and that the factor is not the egg-laying hormone (ELH). The bag cell factor does not affect mitochondrial calcium levels in the Lymnaea albumen gland, and synthetic calfluxin does not affect the Aplysia gland. Thus, although the bag cell and caudodorsal cell peptides have the same activity in their respective genera, the sequences have diverged sufficiently during the course of evolution to preclude cross-reactivity. Calfluxin-related activity was also detected in the atrial gland of A. californica and the atrial gland-like epithelium of A. brasiliana, two exocrine organs in the oviduct that express genes structurally related to the bag cell ELH gene. It is postulated that the active atrial gland factors may be peptides A and B.
AB - The caudodorsal cells of Lymnaea and the bag cells of Aplysia are neuroendocrine cells whose peptide products have homologous functions, i.e., regulation of egg deposition. One of the Lymnaea products, calfluxin, increases cytosolic and hence mitochondrial calcium concentrations in secretory cells of the albumen gland, an exocrine organ that secretes perivitellin fluid around the egg cells during packaging; the changes can be visualized at the ultrastructural level for quantification with the pyroantimonate precipitation technique and are correlated with changes in the secretory and biosynthetic activity in the gland. Comparable studies have now been carried out with Aplysia and indicate that the bag cells of A. californica and A. brasiliana also contain a factor with calfluxin-related activity, and that the factor is not the egg-laying hormone (ELH). The bag cell factor does not affect mitochondrial calcium levels in the Lymnaea albumen gland, and synthetic calfluxin does not affect the Aplysia gland. Thus, although the bag cell and caudodorsal cell peptides have the same activity in their respective genera, the sequences have diverged sufficiently during the course of evolution to preclude cross-reactivity. Calfluxin-related activity was also detected in the atrial gland of A. californica and the atrial gland-like epithelium of A. brasiliana, two exocrine organs in the oviduct that express genes structurally related to the bag cell ELH gene. It is postulated that the active atrial gland factors may be peptides A and B.
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U2 - 10.1016/0016-6480(90)90094-3
DO - 10.1016/0016-6480(90)90094-3
M3 - Article
C2 - 2354773
AN - SCOPUS:0025369727
SN - 0016-6480
VL - 79
SP - 114
EP - 122
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
IS - 1
ER -