A chemical phosphorylation-inspired design for Type i collagen biomimetic remineralization

Li Sha Gu, Jongryul Kim, Young Kyung Kim, Yan Liu, Sabine H. Dickens, David Henry Pashley, Jun Qi Ling, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objectives: Type I collagen alone cannot initiate tissue mineralization. Sodium trimetaphosphate (STMP) is frequently employed as a chemical phosphorylating reagent in the food industry. This study examined the feasibility of using STMP as a functional analog of matrix phosphoproteins for biomimetic remineralization of resin-bonded dentin. Methods: Equilibrium adsorption and desorption studies of STMP were performed using demineralized dentin powder (DDP). Interaction between STMP and DDP was examined using Fourier transform-infrared spectroscopy. Based on those results, a bio-inspired mineralization scheme was developed for chemical phosphorylation of acid-etched dentin with STMP, followed by infiltration of the STMP-treated collagen matrix with two etch-and-rinse adhesives. Resin-dentin interfaces were remineralized in a Portland cement-simulated body fluid system, with or without the use of polyacrylic acid (PAA) as a dual biomimetic analog. Remineralized resin-dentin interfaces were examined unstained using transmission electron microscopy. Results: Analysis of saturation binding curves revealed the presence of irreversible phosphate group binding sites on the surface of the DDP. FT-IR provided additional evidence of chemical interaction between STMP and DDP, with increased in the peak intensities of the PO and P-O-C stretching modes. Those peaks returned to their original intensities after alkaline phosphatase treatment. Evidence of intrafibrillar apatite formation could be seen in incompletely resin-infiltrated, STMP-phosphorylated collagen matrices only when PAA was present in the SBF. Significance: These results reinforce the importance of PAA for sequestration of amorphous calcium phosphate nanoprecursors in the biomimetic remineralization scheme. They also highlight the role of STMP as a templating analog of dentin matrix phosphoproteins for inducing intrafibrillar remineralization of apatite nanocrystals within the collagen matrix of incompletely resin-infiltrated dentin.

Original languageEnglish (US)
Pages (from-to)1077-1089
Number of pages13
JournalDental Materials
Volume26
Issue number11
DOIs
StatePublished - Nov 1 2010

Fingerprint

Biomimetics
Phosphorylation
Dentin
Collagen
carbopol 940
Sodium
Resins
Powders
Apatites
Acids
Phosphoproteins
Apatite
Biomineralization
trimetaphosphoric acid
Food Industry
Body fluids
Phosphatases
Calcium phosphate
Feasibility Studies
Body Fluids

Keywords

  • Adsorption
  • Collagen
  • Dentin
  • Intrafibrillar remineralization
  • Sodium trimetaphosphate

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

A chemical phosphorylation-inspired design for Type i collagen biomimetic remineralization. / Gu, Li Sha; Kim, Jongryul; Kim, Young Kyung; Liu, Yan; Dickens, Sabine H.; Pashley, David Henry; Ling, Jun Qi; Tay, Franklin Chi Meng.

In: Dental Materials, Vol. 26, No. 11, 01.11.2010, p. 1077-1089.

Research output: Contribution to journalArticle

Gu, LS, Kim, J, Kim, YK, Liu, Y, Dickens, SH, Pashley, DH, Ling, JQ & Tay, FCM 2010, 'A chemical phosphorylation-inspired design for Type i collagen biomimetic remineralization', Dental Materials, vol. 26, no. 11, pp. 1077-1089. https://doi.org/10.1016/j.dental.2010.07.008
Gu, Li Sha ; Kim, Jongryul ; Kim, Young Kyung ; Liu, Yan ; Dickens, Sabine H. ; Pashley, David Henry ; Ling, Jun Qi ; Tay, Franklin Chi Meng. / A chemical phosphorylation-inspired design for Type i collagen biomimetic remineralization. In: Dental Materials. 2010 ; Vol. 26, No. 11. pp. 1077-1089.
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AU - Pashley, David Henry

AU - Ling, Jun Qi

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