A chemical phosphorylation-inspired design for Type i collagen biomimetic remineralization

Li Sha Gu, Jongryul Kim, Young Kyung Kim, Yan Liu, Sabine H. Dickens, David H. Pashley, Jun Qi Ling, Franklin R. Tay

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Objectives: Type I collagen alone cannot initiate tissue mineralization. Sodium trimetaphosphate (STMP) is frequently employed as a chemical phosphorylating reagent in the food industry. This study examined the feasibility of using STMP as a functional analog of matrix phosphoproteins for biomimetic remineralization of resin-bonded dentin. Methods: Equilibrium adsorption and desorption studies of STMP were performed using demineralized dentin powder (DDP). Interaction between STMP and DDP was examined using Fourier transform-infrared spectroscopy. Based on those results, a bio-inspired mineralization scheme was developed for chemical phosphorylation of acid-etched dentin with STMP, followed by infiltration of the STMP-treated collagen matrix with two etch-and-rinse adhesives. Resin-dentin interfaces were remineralized in a Portland cement-simulated body fluid system, with or without the use of polyacrylic acid (PAA) as a dual biomimetic analog. Remineralized resin-dentin interfaces were examined unstained using transmission electron microscopy. Results: Analysis of saturation binding curves revealed the presence of irreversible phosphate group binding sites on the surface of the DDP. FT-IR provided additional evidence of chemical interaction between STMP and DDP, with increased in the peak intensities of the PO and P-O-C stretching modes. Those peaks returned to their original intensities after alkaline phosphatase treatment. Evidence of intrafibrillar apatite formation could be seen in incompletely resin-infiltrated, STMP-phosphorylated collagen matrices only when PAA was present in the SBF. Significance: These results reinforce the importance of PAA for sequestration of amorphous calcium phosphate nanoprecursors in the biomimetic remineralization scheme. They also highlight the role of STMP as a templating analog of dentin matrix phosphoproteins for inducing intrafibrillar remineralization of apatite nanocrystals within the collagen matrix of incompletely resin-infiltrated dentin.

Original languageEnglish (US)
Pages (from-to)1077-1089
Number of pages13
JournalDental Materials
Volume26
Issue number11
DOIs
StatePublished - Nov 2010

Keywords

  • Adsorption
  • Collagen
  • Dentin
  • Intrafibrillar remineralization
  • Sodium trimetaphosphate

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

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    Gu, L. S., Kim, J., Kim, Y. K., Liu, Y., Dickens, S. H., Pashley, D. H., Ling, J. Q., & Tay, F. R. (2010). A chemical phosphorylation-inspired design for Type i collagen biomimetic remineralization. Dental Materials, 26(11), 1077-1089. https://doi.org/10.1016/j.dental.2010.07.008