Abstract
A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.
Original language | English (US) |
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Pages (from-to) | 107-110 |
Number of pages | 4 |
Journal | Lipids |
Volume | 17 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1982 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Cell Biology