A convenient method for the preparation of asialo-GM1

Noriyuki Kasai, Laurel O. Sillerud, Robert K. Yu

Research output: Contribution to journalArticlepeer-review

44 Scopus citations


A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
Issue number2
StatePublished - Feb 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology


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