A convenient method for the preparation of asialo-GM1

Noriyuki Kasai, Laurel O. Sillerud, Robert K. Yu

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalLipids
Volume17
Issue number2
DOIs
StatePublished - Feb 1 1982

Fingerprint

formic acid
Hydrolysis
Brain
Column chromatography
Gangliosides
Chromatography
asialo GM1 ganglioside

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

A convenient method for the preparation of asialo-GM1. / Kasai, Noriyuki; Sillerud, Laurel O.; Yu, Robert K.

In: Lipids, Vol. 17, No. 2, 01.02.1982, p. 107-110.

Research output: Contribution to journalArticle

Kasai, Noriyuki ; Sillerud, Laurel O. ; Yu, Robert K. / A convenient method for the preparation of asialo-GM1. In: Lipids. 1982 ; Vol. 17, No. 2. pp. 107-110.
@article{7baba41ce4bf4b41b70527e009c3cae1,
title = "A convenient method for the preparation of asialo-GM1",
abstract = "A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50{\%}.",
author = "Noriyuki Kasai and Sillerud, {Laurel O.} and Yu, {Robert K.}",
year = "1982",
month = "2",
day = "1",
doi = "10.1007/BF02535184",
language = "English (US)",
volume = "17",
pages = "107--110",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - A convenient method for the preparation of asialo-GM1

AU - Kasai, Noriyuki

AU - Sillerud, Laurel O.

AU - Yu, Robert K.

PY - 1982/2/1

Y1 - 1982/2/1

N2 - A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.

AB - A convenient and efficient procedure has been devised for the large-scale preparation of asialo-GM1 from bovine brain gangliosides. The procedure relies on the complete desialylation of brain ganglio-sides, consisting primarily of GM1, GD1a, GD1b and GT1b, by mild formic acid hydrolysis (0.1 N, 100 C; 2 hr). Following the hydrolysis step, asialo-GM1 can be isolated and purified by Folch partitioning and Iatrobeads column chromatography, with an overall yield of more than 50%.

UR - http://www.scopus.com/inward/record.url?scp=0020093397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020093397&partnerID=8YFLogxK

U2 - 10.1007/BF02535184

DO - 10.1007/BF02535184

M3 - Article

C2 - 7087683

AN - SCOPUS:0020093397

VL - 17

SP - 107

EP - 110

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 2

ER -