A crucial role for cAMP and protein kinase A in D1 dopamine receptor regulated intracellular calcium transients

Rujuan Dai, Mohammad K. Ali, Nelson Lezcano, Clare M Bergson

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

D1-like dopamine receptors stimulate Ca 2+ transients in neurons but the effector coupling and signaling mechanisms underlying these responses have not been elucidated. Here we investigated potential mechanisms using both HEK 293 cells that stably express D1 receptors (D1HEK293) and hippocampal neurons in culture. In D1HEK293 cells, the full D1 receptor agonist SKF 81297 evoked a robust dose-dependent increase in Ca 2+ i following 'priming' of endogenous G q/11 -coupled muscarinic or purinergic receptors. The effect of SKF81297 could be mimicked by forskolin or 8-Br-cAMP. Further, cholera toxin and the cAMP-dependent protein kinase (PKA) inhibitors, KT5720 and H89, as well as thapsigargin abrogated the D1 receptor evoked Ca 2+ transients. Removal of the priming agonist and treatment with the phospholipase C inhibitor U73122 also blocked the SKF81297-evoked responses. D1R agonist did not stimulate IP 3 production, but pretreatment of cells with the D1R agonist potentiated G q -linked receptor agonist mobilization of intracellular Ca 2+ stores. In neurons, SKF81297 and SKF83959, a partial D1 receptor agonist, promoted Ca 2+ oscillations in response to G q/11 -coupled metabotropic glutamate receptor (mGluR) stimulation. The effects of both D1R agonists on the mGluR-evoked Ca 2+ responses were PKA dependent. Altogether the data suggest that dopamine D1R activation and ensuing cAMP production dynamically regulates the efficiency and timing of IP 3 -mediated intracellular Ca 2+ store mobilization.

Original languageEnglish (US)
Pages (from-to)112-123
Number of pages12
JournalNeuroSignals
Volume16
Issue number2-3
DOIs
StatePublished - Feb 1 2008

Fingerprint

Dopamine D1 Receptors
Cyclic AMP-Dependent Protein Kinases
Calcium
Metabotropic Glutamate Receptors
Neurons
Purinergic Receptors
Thapsigargin
HEK293 Cells
Cholera Toxin
Type C Phospholipases
Muscarinic Receptors
Colforsin
Protein Kinase Inhibitors
Protein Kinases
Dopamine
SK&F 81297

Keywords

  • Crosstalk
  • G protein-linked receptor
  • Gq
  • Gs
  • IP
  • Phospholipase C
  • Priming
  • Protein kinase A

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

A crucial role for cAMP and protein kinase A in D1 dopamine receptor regulated intracellular calcium transients. / Dai, Rujuan; Ali, Mohammad K.; Lezcano, Nelson; Bergson, Clare M.

In: NeuroSignals, Vol. 16, No. 2-3, 01.02.2008, p. 112-123.

Research output: Contribution to journalArticle

Dai, Rujuan ; Ali, Mohammad K. ; Lezcano, Nelson ; Bergson, Clare M. / A crucial role for cAMP and protein kinase A in D1 dopamine receptor regulated intracellular calcium transients. In: NeuroSignals. 2008 ; Vol. 16, No. 2-3. pp. 112-123.
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