A focused microarray to study human mitochondrial and nuclear gene expression

Joachim G. Voss, Raghavan Raju, Carolea Logun, Robert L. Danner, Peter J. Munson, Zoila Rangel, Marinos C. Dalakas

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A focused microarray (huMITOchip) was developed to study alterations of human mitochondrial and nuclear gene expression in health and disease. The huMITOchip contains 4,774 probe sets identical to the Affymetrix U 133 plus 2.0 chip covering genes affecting mitochondrial, lipid, cytokine, apoptosis, and muscle function transcripts. Unlike other gene chips, the huMITOchip has 51 probe sets that interrogate 37 genes of the mitochondrial genome. The human mitochondrial gene chip was validated against the Affymetrix U133 plus 2.0 array using an in vitro system of CCL136 muscle cell line stimulated with or without interferon gamma (IFN-γ). The 37 genes from the mtDNA demonstrated absolute gene expression levels ranging from 0.1 to 3,182. The comparison of the two gene chips yielded an excellent Pearson's correlation coefficient (r = 0.98). At least 17 probe sets were differentially expressed in response to IFN-γ on both chips, with a high degree of concordance. This is the first report on the development of a focused oligonucleotide microarray containing genes of the mitochondrial genome.

Original languageEnglish (US)
Pages (from-to)272-279
Number of pages8
JournalBiological research for nursing
Volume9
Issue number4
DOIs
StatePublished - Apr 2008

Keywords

  • Gene expression
  • Interferon gamma
  • Microarray
  • Mitochondria

ASJC Scopus subject areas

  • Research and Theory

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