A functional link between localized Oskar, dynamic microtubules, and endocytosis

Paulomi Sanghavi, Sumin Lu, Graydon Bennett Gonsalvez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Many cell types including developing oocytes, fibroblasts, epithelia and neurons use mRNA localization as a means to establish polarity. The Drosophila oocyte has served as a useful model in dissecting the mechanism of mRNA localization. The polarity of the oocyte is established by the specific localization of three critical mRNAs-oskar, bicoid and gurken. The localization of these mRNAs requires microtubule integrity, and the activity of microtubule motors. However, the precise organization of the oocyte microtubule cytoskeleton remains an open question. In order to examine the polarity of oocyte microtubules, we visualized the localization of canonical microtubule plus end binding proteins, EB1 and CLIP-190. Both proteins were enriched at the posterior of the oocyte, with additional foci detected within the oocyte cytoplasm and along the cortex. Surprisingly, however, we found that this asymmetric distribution of EB1 and CLIP-190 was not essential for oskar mRNA localization. However, Oskar protein was required for recruiting the plus end binding proteins to the oocyte posterior. Lastly, our results suggest that the enrichment of growing microtubules at the posterior pole functions to promote high levels of endocytosis in this region of the cell. Thus, multiple polarity-determining pathways are functionally linked in the Drosophila oocytes.

Original languageEnglish (US)
Pages (from-to)66-77
Number of pages12
JournalDevelopmental Biology
Volume367
Issue number1
DOIs
StatePublished - Jul 1 2012

Fingerprint

Endocytosis
Microtubules
Oocytes
Messenger RNA
Drosophila
Carrier Proteins
Cytoskeleton
Cytoplasm
Proteins
Motor Activity
Epithelium
Fibroblasts
Neurons

Keywords

  • Actin anchoring
  • Cell polarity
  • Kinesin
  • RNA localization

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

A functional link between localized Oskar, dynamic microtubules, and endocytosis. / Sanghavi, Paulomi; Lu, Sumin; Gonsalvez, Graydon Bennett.

In: Developmental Biology, Vol. 367, No. 1, 01.07.2012, p. 66-77.

Research output: Contribution to journalArticle

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