TY - JOUR
T1 - A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide
AU - Nagle, Gregg T.
AU - Greenberg, Michael J.
N1 - Funding Information:
Acknow/edyements This work was supported by NIH Grant HL-09283 to M.J.G. and is Contribution No. 163 from the Tallahassee, Sopchoppy & Gulf Coast Marine Biological Association. We thank James Hogg and John Highsmith for constructing the microbath, Nancy Hopkins for the mechanical drawing and Dr John A. Watts for the microbath photograph. Dr David Price provided helpful suggestions in the original design.
PY - 1982
Y1 - 1982
N2 - 1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.
AB - 1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.
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U2 - 10.1016/0306-4492(82)90017-X
DO - 10.1016/0306-4492(82)90017-X
M3 - Article
AN - SCOPUS:0020041429
SN - 0306-4492
VL - 71
SP - 101
EP - 105
JO - Comparative Biochemistry and Physiology. Part C, Comparative
JF - Comparative Biochemistry and Physiology. Part C, Comparative
IS - 1
ER -