A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide

Gregg T. Nagle; Michael J. Greenberg

doi:10.1016/0306-4492(82)90017-X

A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide

Gregg T. Nagle, Michael J. Greenberg

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.

Original language	English (US)
Pages (from-to)	101-105
Number of pages	5
Journal	Comparative Biochemistry and Physiology. Part C, Comparative
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/0306-4492(82)90017-X
State	Published - 1982
Externally published	Yes

ASJC Scopus subject areas

Immunology
Pharmacology

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title = "A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide",

abstract = "1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.",

author = "Nagle, {Gregg T.} and Greenberg, {Michael J.}",

note = "Funding Information: Acknow/edyements This work was supported by NIH Grant HL-09283 to M.J.G. and is Contribution No. 163 from the Tallahassee, Sopchoppy & Gulf Coast Marine Biological Association. We thank James Hogg and John Highsmith for constructing the microbath, Nancy Hopkins for the mechanical drawing and Dr John A. Watts for the microbath photograph. Dr David Price provided helpful suggestions in the original design.",

year = "1982",

doi = "10.1016/0306-4492(82)90017-X",

language = "English (US)",

volume = "71",

pages = "101--105",

journal = "Comparative biochemistry and physiology. C: Comparative pharmacology",

issn = "0306-4492",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide

AU - Nagle, Gregg T.

AU - Greenberg, Michael J.

N1 - Funding Information: Acknow/edyements This work was supported by NIH Grant HL-09283 to M.J.G. and is Contribution No. 163 from the Tallahassee, Sopchoppy & Gulf Coast Marine Biological Association. We thank James Hogg and John Highsmith for constructing the microbath, Nancy Hopkins for the mechanical drawing and Dr John A. Watts for the microbath photograph. Dr David Price provided helpful suggestions in the original design.

PY - 1982

Y1 - 1982

N2 - 1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.

AB - 1. An organ bath of very small volume (100 μl) has been designed for the superfusion of long thin molluscan muscles. The plan of this microbath is presented in detail. 2. Isolated in the microbath, radula protractor muscles from small specimens of the lightning whelk (Busycon contrarium) can be used to assay as little as 0.1 pmols of the molluscan neuropeptide FMRFamide.

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U2 - 10.1016/0306-4492(82)90017-X

DO - 10.1016/0306-4492(82)90017-X

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VL - 71

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EP - 105

JO - Comparative biochemistry and physiology. C: Comparative pharmacology

JF - Comparative biochemistry and physiology. C: Comparative pharmacology

SN - 0306-4492

IS - 1

ER -

A highly sensitive microbioassay for the molluscan neuropeptide FMRFamide

Abstract

ASJC Scopus subject areas

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