A human IgM M-protein in a patient with unknown bleeding disorder binds to β-galactosyl and β-glucosyl residues

Susumu Kusunoki, Joseph E. Craft, Barbara Roach, John A. Hardin, Robert K Yu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In a patient with an unknown bleeding disorder and an IgM λ paraproteinemia, we demonstrated by thin-layer chromatography immunostaining and enzyme-linked immunosorbent assay that this protein specifically bound to a number of glycolipids and glycoproteins which have terminal β-galactosyl or β-glucosyl residues. Binding to galactosylceramide or glucosylceramide was inhibited by both galactosylceramide and glucosylceramide. From these studies, it is apparent that the M-protein recognized both β-galactosyl and β-glucosyl residues. This M-protein was also shown to prolong the partial thromboplastin time of normal plasma. Thus, this case represents an example of anti-carbohydrate specificity of an IgM M-protein in association with a spontaneous bleeding disorder.

Original languageEnglish (US)
Pages (from-to)226-232
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume255
Issue number2
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

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Immunoglobulin M
Galactosylceramides
Hemorrhage
Glucosylceramides
Proteins
Thin layer chromatography
Immunosorbents
Paraproteinemias
Partial Thromboplastin Time
Glycolipids
Thromboplastin
Thin Layer Chromatography
Assays
Glycoproteins
Enzyme-Linked Immunosorbent Assay
Carbohydrates
Association reactions
Plasmas
Enzymes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

A human IgM M-protein in a patient with unknown bleeding disorder binds to β-galactosyl and β-glucosyl residues. / Kusunoki, Susumu; Craft, Joseph E.; Roach, Barbara; Hardin, John A.; Yu, Robert K.

In: Archives of Biochemistry and Biophysics, Vol. 255, No. 2, 01.01.1987, p. 226-232.

Research output: Contribution to journalArticle

Kusunoki, Susumu ; Craft, Joseph E. ; Roach, Barbara ; Hardin, John A. ; Yu, Robert K. / A human IgM M-protein in a patient with unknown bleeding disorder binds to β-galactosyl and β-glucosyl residues. In: Archives of Biochemistry and Biophysics. 1987 ; Vol. 255, No. 2. pp. 226-232.
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