A key role for Na+/K+-ATPase in the endothelium-dependent oscillatory activity of mouse small mesenteric arteries

F. R.C. Giachini, F. S. Carneiro, V. V. Lima, Z. N. Carneiro, M. W. Brands, R. C. Webb, R. C. Tostes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Oscillatory contractile activity is an inherent property of blood vessels. Various cellular mechanisms have been proposed to contribute to oscillatory activity. Mouse small mesenteric arteries display a unique low frequency contractile oscillatory activity (1 cycle every 10-12 min) upon phenylephrine stimulation. Our objective was to identify mechanisms involved in this peculiar oscillatory activity. First-order mesenteric arteries were mounted in tissue baths for isometric force measurement. The oscillatory activity was observed only in vessels with endothelium, but it was not blocked by L-NAME (100 μM) or indomethacin (10 μM), ruling out the participation of nitric oxide and prostacyclin, respectively, in this phenomenon. Oscillatory activity was not observed in vessels contracted with K+ (90 mM) or after stimulation with phenylephrine plus 10 mM K+. Ouabain (1 to 10 μM, an Na +/K+-ATPase inhibitor), but not K+ channel antagonists [tetraethylammonium (100 μM, a nonselective K+ channel blocker), Tram-34 (10 μM, blocker of intermediate conductance K+ channels) or UCL-1684 (0.1 μM, a small conductance K+ channel blocker)], inhibited the oscillatory activity. The contractile activity was also abolished when experiments were performed at 20°C or in K+-free medium. Taken together, these results demonstrate that Na+/K +-ATPase is a potential source of these oscillations. The presence of α-1 and α-2 Na+/K+-ATPase isoforms was confirmed in murine mesenteric arteries by Western blot. Chronic infusion of mice with ouabain did not abolish oscillatory contraction, but up-regulated vascular Na+/K+-ATPase expression and increased blood pressure. Together, these observations suggest that the Na+/K + pump plays a major role in the oscillatory activity of murine small mesenteric arteries.

Original languageEnglish (US)
Pages (from-to)1058-1067
Number of pages10
JournalBrazilian Journal of Medical and Biological Research
Volume42
Issue number11
DOIs
StatePublished - Dec 8 2009

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Mesenteric Arteries
Endothelium
Adenosine Triphosphatases
Phenylephrine
Ouabain
Blood Vessels
Tetraethylammonium
Force measurement
NG-Nitroarginine Methyl Ester
Blood pressure
Blood vessels
Epoprostenol
Activity Cycles
Indomethacin
Nitric Oxide
Protein Isoforms
Baths
Pumps
Tissue
Western Blotting

Keywords

  • Na/K-ATPase pump
  • Oscillatory activity
  • Ouabain
  • Small mesenteric arteries

ASJC Scopus subject areas

  • Biophysics
  • Neuroscience(all)
  • Biochemistry
  • Physiology
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Cell Biology

Cite this

A key role for Na+/K+-ATPase in the endothelium-dependent oscillatory activity of mouse small mesenteric arteries. / Giachini, F. R.C.; Carneiro, F. S.; Lima, V. V.; Carneiro, Z. N.; Brands, M. W.; Webb, R. C.; Tostes, R. C.

In: Brazilian Journal of Medical and Biological Research, Vol. 42, No. 11, 08.12.2009, p. 1058-1067.

Research output: Contribution to journalArticle

Giachini, F. R.C. ; Carneiro, F. S. ; Lima, V. V. ; Carneiro, Z. N. ; Brands, M. W. ; Webb, R. C. ; Tostes, R. C. / A key role for Na+/K+-ATPase in the endothelium-dependent oscillatory activity of mouse small mesenteric arteries. In: Brazilian Journal of Medical and Biological Research. 2009 ; Vol. 42, No. 11. pp. 1058-1067.
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AU - Carneiro, F. S.

AU - Lima, V. V.

AU - Carneiro, Z. N.

AU - Brands, M. W.

AU - Webb, R. C.

AU - Tostes, R. C.

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