A molecular mimic demonstrates that phosphorylated human prolactin is a potent anti-angiogenic hormone

Eric Ueda, Ugur Ozerdem, Yen-Hao Chen, Min Yao, Tzu Huang Kuang, Huiqin Sun, Manuela Martins-Green, Paolo Bartolini, Ameae M. Walker

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

S179D prolactin (PRL) is an experimentally useful mimic of naturally phosphorylated human prolactin. S179D PRL, but not unmodified PRL, was found to be anti-angiogenic in both the chorioallantoic membrane and corneal assays. Further investigation using human endothelial in vitro models showed reduced cell number, reduced tubule formation in Matrigel, and reduced migration and invasion, as a function of treatment with S179D PRL. Analysis of growth factors in human endothelial cells in response to S179D PRL showed: a decreased expression or release of endogenous PRL, heme-oxygenase-1, basic fibroblast growth factor (bFGF), angiogenin, epidermal growth factor and vascular endothelial growth factor; and an increased expression of inhibitors of matrix metalloproteases. S179D PRL also blocked signaling from bFGF in these cells. We conclude that this molecular mimic of a pituitary hormone is a potent anti-angiogenic protein, partly as a result of its ability to reduce utilization of several well-established endothelial autocrine growth loops, partly by its ability to block signaling from bFGF and partly because of its ability to decrease endothelial migration. These findings suggest that circulating levels of phosphorylated PRL may influence the progression of cancer and, furthermore, that S179D PRL may be a useful anti-angiogenic therapeutic.

Original languageEnglish (US)
Pages (from-to)95-111
Number of pages17
JournalEndocrine-Related Cancer
Volume13
Issue number1
DOIs
StatePublished - Mar 1 2006

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Oncology
  • Endocrinology
  • Cancer Research

Fingerprint Dive into the research topics of 'A molecular mimic demonstrates that phosphorylated human prolactin is a potent anti-angiogenic hormone'. Together they form a unique fingerprint.

  • Cite this

    Ueda, E., Ozerdem, U., Chen, Y-H., Yao, M., Kuang, T. H., Sun, H., Martins-Green, M., Bartolini, P., & Walker, A. M. (2006). A molecular mimic demonstrates that phosphorylated human prolactin is a potent anti-angiogenic hormone. Endocrine-Related Cancer, 13(1), 95-111. https://doi.org/10.1677/erc.1.01076