A Monoclonal Autoantibody against a Complementary Peptide Recognizes Interstitial Collagenase

Sandro José de Souza, Michael P. Madaio, Luis Juliano Neto, Ricardo Renzo Brentani

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.

Original languageEnglish (US)
Pages (from-to)172-176
Number of pages5
JournalImmunoMethods
Volume5
Issue number2
DOIs
StatePublished - Oct 1 1994

ASJC Scopus subject areas

  • Immunology

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