TY - JOUR
T1 - A Monoclonal Autoantibody against a Complementary Peptide Recognizes Interstitial Collagenase
AU - de Souza, Sandro José
AU - Madaio, Michael P.
AU - Neto, Luis Juliano
AU - Brentani, Ricardo Renzo
PY - 1994/10/1
Y1 - 1994/10/1
N2 - A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.
AB - A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.
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U2 - 10.1006/immu.1994.1052
DO - 10.1006/immu.1994.1052
M3 - Article
C2 - 7874441
AN - SCOPUS:0028610175
VL - 5
SP - 172
EP - 176
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - 2
ER -