A Monoclonal Autoantibody against a Complementary Peptide Recognizes Interstitial Collagenase

Sandro José de Souza; Michael P. Madaio; Luis Juliano Neto; Ricardo Renzo Brentani

doi:10.1006/immu.1994.1052

A Monoclonal Autoantibody against a Complementary Peptide Recognizes Interstitial Collagenase

Sandro José de Souza, Michael P. Madaio, Luis Juliano Neto, Ricardo Renzo Brentani

Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.

Original language	English (US)
Pages (from-to)	172-176
Number of pages	5
Journal	ImmunoMethods
Volume	5
Issue number	2
DOIs	https://doi.org/10.1006/immu.1994.1052
State	Published - Oct 1 1994

ASJC Scopus subject areas

Immunology

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abstract = "A monoclonal autoantibody (mab 16) against the complementary peptide TKKTLRT, which was deduced from the collagenase-sensitive site in collagen, is described. Mab 16 recognized interstitial collagenase as visualized by ELISA and immunoprecipitation assays. Moreover, mab 16 was able to inhibit partially the collagenolytic activity of keratinocyte supernatant. Finally, it was possible to immunopurify collagenase using a mab 16/Sepharose column.",

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