A mouse model of the cornea pocket assay for angiogenesis study

Zhongshu Tang, Fan Zhang, Yang Li, Pachiappan Arjunan, Anil Kumar, Chunsik Lee, Xuri Li

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A normal cornea is clear of vascular tissues. However, blood vessels can be induced to grow and survive in the cornea when potent angiogenic factors are administered 1. This uniqueness has made the cornea pocket assay one of the most used models for angiogenesis studies. The cornea composes multiple layers of cells. It is therefore possible to embed a pellet containing the angiogenic factor of interest in the cornea to investigate its angiogenic effect 2,3. Here, we provide a step by step demonstration of how to (I) produce the angiogenic factor-containing pellet (II) embed the pellet into the cornea (III) analyze the angiogenesis induced by the angiogenic factor of interest. Since the basic fibroblast growth factor (bFGF) is known as one of the most potent angiogenic factors 4, it is used here to induce angiogenesis in the cornea.

Original languageEnglish (US)
Article numbere3077
JournalJournal of Visualized Experiments
Issue number54
DOIs
StatePublished - Aug 1 2011

Fingerprint

Angiogenesis Inducing Agents
Blood vessels
Fibroblasts
Cornea
Assays
Demonstrations
Tissue
Blood Vessels
Fibroblast Growth Factor 2
Intercellular Signaling Peptides and Proteins

Keywords

  • Angiogenesis
  • Issue 54
  • Medicine
  • Mouse cornea pocket assay

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A mouse model of the cornea pocket assay for angiogenesis study. / Tang, Zhongshu; Zhang, Fan; Li, Yang; Arjunan, Pachiappan; Kumar, Anil; Lee, Chunsik; Li, Xuri.

In: Journal of Visualized Experiments, No. 54, e3077, 01.08.2011.

Research output: Contribution to journalArticle

Tang, Zhongshu ; Zhang, Fan ; Li, Yang ; Arjunan, Pachiappan ; Kumar, Anil ; Lee, Chunsik ; Li, Xuri. / A mouse model of the cornea pocket assay for angiogenesis study. In: Journal of Visualized Experiments. 2011 ; No. 54.
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