A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph

Man Xu, Xue Wang, Juan Tan, Kui Zhang, Xi Guan, Laurence H. Patterson, Hanfei Ding, Hongjuan Cui

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Runt-related (RUNX) transcription factors are evolutionarily conserved either in vertebrate or invertebrate. Lozenge (Lz), a members of RUNX family as well as homologue of AML-1, functions as an important transcription factor regulating the hemocytes differentiation. In this paper, we identified and characterized RUNX family especially Lz in silkworm, which is a lepidopteran model insect. The gene expression analysis illustrated that BmLz was highly expressed in hemocytes throughout the whole development period, and reached a peak in glutonous stage. Over-expression of BmLz in silkworm accelerated the melanization process of hemolymph, and led to instantaneously up-regulation of prophenoloxidases (PPOs), which were key enzymes in the melanization process. Further down-regulation of BmLz expression by RNA interference resulted in the significant delay of melanization reaction of hemolymph. These findings suggested that BmLz regulated the melanization process of hemolymph by inducing PPOs expression, and played a critical role in innate immunity defense in silkworm.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume53
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Bombyx
Hemolymph
Hemocytes
Transcription Factors
Genes
Invertebrates
RNA Interference
Innate Immunity
Insects
Vertebrates
Up-Regulation
Down-Regulation
Gene Expression
Enzymes

Keywords

  • Bombyx mori
  • Lozenge
  • Melanization
  • Prophenoloxidases
  • Runt-related transcription factor (RUNX)

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph. / Xu, Man; Wang, Xue; Tan, Juan; Zhang, Kui; Guan, Xi; Patterson, Laurence H.; Ding, Hanfei; Cui, Hongjuan.

In: Developmental and Comparative Immunology, Vol. 53, No. 1, 01.01.2015, p. 191-198.

Research output: Contribution to journalArticle

Xu, Man ; Wang, Xue ; Tan, Juan ; Zhang, Kui ; Guan, Xi ; Patterson, Laurence H. ; Ding, Hanfei ; Cui, Hongjuan. / A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph. In: Developmental and Comparative Immunology. 2015 ; Vol. 53, No. 1. pp. 191-198.
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