TY - JOUR
T1 - A novel Lozenge gene in silkworm, Bombyx mori regulates the melanization response of hemolymph
AU - Xu, Man
AU - Wang, Xue
AU - Tan, Juan
AU - Zhang, Kui
AU - Guan, Xi
AU - Patterson, Laurence H.
AU - Ding, Hanfei
AU - Cui, Hongjuan
N1 - Funding Information:
This work was supported by the National Basic Research Program of China ( 2012CB114603 ), the Research Fund for the Doctoral Program of Higher Education of China ( 20130182110003 ), the Natural Science Foundation of Chongqing ( CSTC2013jcyjys0007 ), and the Fundamental Research Funds for the Central Universities ( SWU111014 , XDJK2015D021 ). We are grateful to Prof. Chunfeng Li of the State Key Laboratory of Silkworm Genome Biology for his help in preparation of this manuscript.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2015
Y1 - 2015
N2 - Runt-related (RUNX) transcription factors are evolutionarily conserved either in vertebrate or invertebrate. Lozenge (Lz), a members of RUNX family as well as homologue of AML-1, functions as an important transcription factor regulating the hemocytes differentiation. In this paper, we identified and characterized RUNX family especially Lz in silkworm, which is a lepidopteran model insect. The gene expression analysis illustrated that BmLz was highly expressed in hemocytes throughout the whole development period, and reached a peak in glutonous stage. Over-expression of BmLz in silkworm accelerated the melanization process of hemolymph, and led to instantaneously up-regulation of prophenoloxidases (PPOs), which were key enzymes in the melanization process. Further down-regulation of BmLz expression by RNA interference resulted in the significant delay of melanization reaction of hemolymph. These findings suggested that BmLz regulated the melanization process of hemolymph by inducing PPOs expression, and played a critical role in innate immunity defense in silkworm.
AB - Runt-related (RUNX) transcription factors are evolutionarily conserved either in vertebrate or invertebrate. Lozenge (Lz), a members of RUNX family as well as homologue of AML-1, functions as an important transcription factor regulating the hemocytes differentiation. In this paper, we identified and characterized RUNX family especially Lz in silkworm, which is a lepidopteran model insect. The gene expression analysis illustrated that BmLz was highly expressed in hemocytes throughout the whole development period, and reached a peak in glutonous stage. Over-expression of BmLz in silkworm accelerated the melanization process of hemolymph, and led to instantaneously up-regulation of prophenoloxidases (PPOs), which were key enzymes in the melanization process. Further down-regulation of BmLz expression by RNA interference resulted in the significant delay of melanization reaction of hemolymph. These findings suggested that BmLz regulated the melanization process of hemolymph by inducing PPOs expression, and played a critical role in innate immunity defense in silkworm.
KW - Bombyx mori
KW - Lozenge
KW - Melanization
KW - Prophenoloxidases
KW - Runt-related transcription factor (RUNX)
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U2 - 10.1016/j.dci.2015.07.001
DO - 10.1016/j.dci.2015.07.001
M3 - Article
C2 - 26164197
AN - SCOPUS:84938062050
SN - 0145-305X
VL - 53
SP - 191
EP - 198
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
IS - 1
ER -