A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis

Shinji Matsuda, Alexandru Movila, Maiko Suzuki, Mikihito Kajiya, Wichaya Wisitrasameewong, Rayyan Kayal, Josefine Hirshfeld, Ayman Al-Dharrab, Irma J Savitri, Abdulghani Mira, Hidemi Kurihara, Martin A Taubman, Toshihisa Kawai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.

Original languageEnglish (US)
Pages (from-to)21-25
Number of pages5
JournalJournal of Immunological Methods
Volume438
DOIs
StatePublished - Nov 2016
Externally publishedYes

Fingerprint

Gingival Crevicular Fluid
Periodontitis
Periodontal Diseases
Ligation
Alveolar Bone Loss
Cytokines
Periodontium
Silk
Polysorbates
Exudates and Transudates
Bone Resorption
Interleukin-6

Keywords

  • Alveolar Bone Loss/etiology
  • Animals
  • Biomarkers/analysis
  • Cytokines/analysis
  • Disease Models, Animal
  • Gingival Crevicular Fluid/chemistry
  • Humans
  • Ligation
  • Maxilla/pathology
  • Mice
  • Mice, Inbred C57BL
  • Periodontitis/pathology
  • Specimen Handling/methods

Cite this

A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis. / Matsuda, Shinji; Movila, Alexandru; Suzuki, Maiko; Kajiya, Mikihito; Wisitrasameewong, Wichaya; Kayal, Rayyan; Hirshfeld, Josefine; Al-Dharrab, Ayman; Savitri, Irma J; Mira, Abdulghani; Kurihara, Hidemi; Taubman, Martin A; Kawai, Toshihisa.

In: Journal of Immunological Methods, Vol. 438, 11.2016, p. 21-25.

Research output: Contribution to journalArticle

Matsuda, S, Movila, A, Suzuki, M, Kajiya, M, Wisitrasameewong, W, Kayal, R, Hirshfeld, J, Al-Dharrab, A, Savitri, IJ, Mira, A, Kurihara, H, Taubman, MA & Kawai, T 2016, 'A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis', Journal of Immunological Methods, vol. 438, pp. 21-25. https://doi.org/10.1016/j.jim.2016.08.008
Matsuda, Shinji ; Movila, Alexandru ; Suzuki, Maiko ; Kajiya, Mikihito ; Wisitrasameewong, Wichaya ; Kayal, Rayyan ; Hirshfeld, Josefine ; Al-Dharrab, Ayman ; Savitri, Irma J ; Mira, Abdulghani ; Kurihara, Hidemi ; Taubman, Martin A ; Kawai, Toshihisa. / A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis. In: Journal of Immunological Methods. 2016 ; Vol. 438. pp. 21-25.
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abstract = "Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.",
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T1 - A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis

AU - Matsuda, Shinji

AU - Movila, Alexandru

AU - Suzuki, Maiko

AU - Kajiya, Mikihito

AU - Wisitrasameewong, Wichaya

AU - Kayal, Rayyan

AU - Hirshfeld, Josefine

AU - Al-Dharrab, Ayman

AU - Savitri, Irma J

AU - Mira, Abdulghani

AU - Kurihara, Hidemi

AU - Taubman, Martin A

AU - Kawai, Toshihisa

N1 - Copyright © 2016. Published by Elsevier B.V.

PY - 2016/11

Y1 - 2016/11

N2 - Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.

AB - Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.

KW - Alveolar Bone Loss/etiology

KW - Animals

KW - Biomarkers/analysis

KW - Cytokines/analysis

KW - Disease Models, Animal

KW - Gingival Crevicular Fluid/chemistry

KW - Humans

KW - Ligation

KW - Maxilla/pathology

KW - Mice

KW - Mice, Inbred C57BL

KW - Periodontitis/pathology

KW - Specimen Handling/methods

U2 - 10.1016/j.jim.2016.08.008

DO - 10.1016/j.jim.2016.08.008

M3 - Article

VL - 438

SP - 21

EP - 25

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

ER -