A novel method to analyze leukocyte rolling behavior in vivo

Jessica L. Dunne, Adam P. Goobic, Scott T. Acton, Klaus Ley

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Leukocyte endothelial cell interaction is a fundamentally important process in many disease states. Current methods to analyze such interactions include the parallel-plate flow chamber and intravital microscopy. Here, we present an improvement of the traditional intravital microscopy that allows leukocyte-endothelial cell interaction to be studied from the time the leukocyte makes its initial contact with the endothelium until it adheres to or detaches from the endothelium. The leukocyte is tracked throughout the venular tree with the aid of a motorized stage and the rolling and adhesive behavior is measured off-line. Because this method can involve human error, methods to automate the tracking procedure have been developed. This novel tracking method allows for a more detailed examination of leukocyte-endothelial cell interactions.

Original languageEnglish (US)
Pages (from-to)173-179
Number of pages7
JournalBiological Procedures Online
Volume6
Issue number1
DOIs
StatePublished - Aug 27 2004
Externally publishedYes

Keywords

  • Animals
  • Cell adhesion
  • Leukocytes
  • Mice, knockout
  • Tumor necorisis factor
  • Venules

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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