A novel two-step procedure to expand cardiac Sca-1+ cells clonally

Yao Liang Tang, Leping Shen, Keping Qian, M. Ian Phillips

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Resident cardiac stem cells (CSCs) are characterized by their capacity to self-renew in culture, and are multipotent for forming normal cell types in hearts. CSCs were originally isolated directly from enzymatically digested hearts using stem cell markers. However, long exposure to enzymatic digestion can affect the integrity of stem cell markers on the cell surface, and also compromise stem cell function. Alternatively resident CSCs can migrate from tissue explant and form cardiospheres in culture. However, fibroblast contamination can easily occur during CSC culture. To avoid these problems, we developed a two-step procedure by growing the cells before selecting the Sca-1+ cells and culturing in cardiac fibroblast conditioned medium, they avoid fibroblast overgrowth.

Original languageEnglish (US)
Pages (from-to)877-883
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume359
Issue number4
DOIs
StatePublished - Aug 10 2007
Externally publishedYes

Fingerprint

Stem cells
Stem Cells
Fibroblasts
Cell culture
Conditioned Culture Medium
Digestion
Contamination
Cell Culture Techniques
Tissue

Keywords

  • Cell transplantation
  • Differentiation
  • Myocardial infarction
  • Stem cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A novel two-step procedure to expand cardiac Sca-1+ cells clonally. / Tang, Yao Liang; Shen, Leping; Qian, Keping; Phillips, M. Ian.

In: Biochemical and Biophysical Research Communications, Vol. 359, No. 4, 10.08.2007, p. 877-883.

Research output: Contribution to journalArticle

Tang, Yao Liang ; Shen, Leping ; Qian, Keping ; Phillips, M. Ian. / A novel two-step procedure to expand cardiac Sca-1+ cells clonally. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 359, No. 4. pp. 877-883.
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