A photoactivable source of relaxing factor in genetic hypertension

John R. Charpie, Anthony Peters, R Clinton Webb

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Deendothelialized rings of rabbit aorta relax after exposure to UV light because of release of a relaxing factor that is similar if not identical to nitric oxide. We tested the hypothesis that production of the photo-induced relaxing factor is impaired in a rat model of genetic hypertension. Thoracic aortas were removed from adult Wistar-Kyoto rats and stroke-prone spontaneously hypertensive rats. The vessels were cut into rings, denuded of endothelium, and placed in a muscle bath for isometric force measurement. Rings were contracted with phenylephrine, and relaxation was measured after exposure to UV light. Aortic rings from stroke-prone spontaneously hypertensive rats relaxed to a greater extent after exposure to UV light than did rings from Wistar-Kyoto rats. An inhibitor of nitric oxide synthase -nitro-L-arginine) greatly potentiated the relaxation responses to light in both strains, and these enhanced relaxations were attenuated by tetraethylammonium chloride, potassium chloride, ouabain, or inhibitors of guanylate cyclase. These results suggest that UV irradiation induces relaxation in aortic smooth muscle that is greater in hypertensive than normotensive rats and is greatly enhanced after addition of inhibitors of nitric oxide production. Thus, the unidentified photo-induced relaxing factor is not solely nitric oxide but may also represent either a hyperpolarizing factor, because depolarization blocks the responses entirely, or possibly smooth muscle guanylate cyclase that might itself be photoactivable.

Original languageEnglish (US)
Pages (from-to)894-898
Number of pages5
JournalHypertension
Volume23
Issue number6
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Fingerprint

Ultraviolet Rays
Nitric Oxide
Inbred WKY Rats
Guanylate Cyclase
Inbred SHR Rats
Hypertension
Smooth Muscle
Stroke
Tetraethylammonium
Potassium Chloride
Genetic Models
Phenylephrine
Ouabain
Thoracic Aorta
Baths
Nitric Oxide Synthase
Endothelium
Arginine
Aorta
Rabbits

Keywords

  • Aorta
  • Genetic
  • Hypertension
  • Muscle
  • Nitric oxide
  • Smooth
  • Ultraviolet rays
  • Vascular

ASJC Scopus subject areas

  • Internal Medicine

Cite this

A photoactivable source of relaxing factor in genetic hypertension. / Charpie, John R.; Peters, Anthony; Webb, R Clinton.

In: Hypertension, Vol. 23, No. 6, 01.01.1994, p. 894-898.

Research output: Contribution to journalArticle

Charpie, John R. ; Peters, Anthony ; Webb, R Clinton. / A photoactivable source of relaxing factor in genetic hypertension. In: Hypertension. 1994 ; Vol. 23, No. 6. pp. 894-898.
@article{6fa65936705b418287bc7b51c3fc5f66,
title = "A photoactivable source of relaxing factor in genetic hypertension",
abstract = "Deendothelialized rings of rabbit aorta relax after exposure to UV light because of release of a relaxing factor that is similar if not identical to nitric oxide. We tested the hypothesis that production of the photo-induced relaxing factor is impaired in a rat model of genetic hypertension. Thoracic aortas were removed from adult Wistar-Kyoto rats and stroke-prone spontaneously hypertensive rats. The vessels were cut into rings, denuded of endothelium, and placed in a muscle bath for isometric force measurement. Rings were contracted with phenylephrine, and relaxation was measured after exposure to UV light. Aortic rings from stroke-prone spontaneously hypertensive rats relaxed to a greater extent after exposure to UV light than did rings from Wistar-Kyoto rats. An inhibitor of nitric oxide synthase -nitro-L-arginine) greatly potentiated the relaxation responses to light in both strains, and these enhanced relaxations were attenuated by tetraethylammonium chloride, potassium chloride, ouabain, or inhibitors of guanylate cyclase. These results suggest that UV irradiation induces relaxation in aortic smooth muscle that is greater in hypertensive than normotensive rats and is greatly enhanced after addition of inhibitors of nitric oxide production. Thus, the unidentified photo-induced relaxing factor is not solely nitric oxide but may also represent either a hyperpolarizing factor, because depolarization blocks the responses entirely, or possibly smooth muscle guanylate cyclase that might itself be photoactivable.",
keywords = "Aorta, Genetic, Hypertension, Muscle, Nitric oxide, Smooth, Ultraviolet rays, Vascular",
author = "Charpie, {John R.} and Anthony Peters and Webb, {R Clinton}",
year = "1994",
month = "1",
day = "1",
doi = "10.1161/01.HYP.23.6.894",
language = "English (US)",
volume = "23",
pages = "894--898",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - A photoactivable source of relaxing factor in genetic hypertension

AU - Charpie, John R.

AU - Peters, Anthony

AU - Webb, R Clinton

PY - 1994/1/1

Y1 - 1994/1/1

N2 - Deendothelialized rings of rabbit aorta relax after exposure to UV light because of release of a relaxing factor that is similar if not identical to nitric oxide. We tested the hypothesis that production of the photo-induced relaxing factor is impaired in a rat model of genetic hypertension. Thoracic aortas were removed from adult Wistar-Kyoto rats and stroke-prone spontaneously hypertensive rats. The vessels were cut into rings, denuded of endothelium, and placed in a muscle bath for isometric force measurement. Rings were contracted with phenylephrine, and relaxation was measured after exposure to UV light. Aortic rings from stroke-prone spontaneously hypertensive rats relaxed to a greater extent after exposure to UV light than did rings from Wistar-Kyoto rats. An inhibitor of nitric oxide synthase -nitro-L-arginine) greatly potentiated the relaxation responses to light in both strains, and these enhanced relaxations were attenuated by tetraethylammonium chloride, potassium chloride, ouabain, or inhibitors of guanylate cyclase. These results suggest that UV irradiation induces relaxation in aortic smooth muscle that is greater in hypertensive than normotensive rats and is greatly enhanced after addition of inhibitors of nitric oxide production. Thus, the unidentified photo-induced relaxing factor is not solely nitric oxide but may also represent either a hyperpolarizing factor, because depolarization blocks the responses entirely, or possibly smooth muscle guanylate cyclase that might itself be photoactivable.

AB - Deendothelialized rings of rabbit aorta relax after exposure to UV light because of release of a relaxing factor that is similar if not identical to nitric oxide. We tested the hypothesis that production of the photo-induced relaxing factor is impaired in a rat model of genetic hypertension. Thoracic aortas were removed from adult Wistar-Kyoto rats and stroke-prone spontaneously hypertensive rats. The vessels were cut into rings, denuded of endothelium, and placed in a muscle bath for isometric force measurement. Rings were contracted with phenylephrine, and relaxation was measured after exposure to UV light. Aortic rings from stroke-prone spontaneously hypertensive rats relaxed to a greater extent after exposure to UV light than did rings from Wistar-Kyoto rats. An inhibitor of nitric oxide synthase -nitro-L-arginine) greatly potentiated the relaxation responses to light in both strains, and these enhanced relaxations were attenuated by tetraethylammonium chloride, potassium chloride, ouabain, or inhibitors of guanylate cyclase. These results suggest that UV irradiation induces relaxation in aortic smooth muscle that is greater in hypertensive than normotensive rats and is greatly enhanced after addition of inhibitors of nitric oxide production. Thus, the unidentified photo-induced relaxing factor is not solely nitric oxide but may also represent either a hyperpolarizing factor, because depolarization blocks the responses entirely, or possibly smooth muscle guanylate cyclase that might itself be photoactivable.

KW - Aorta

KW - Genetic

KW - Hypertension

KW - Muscle

KW - Nitric oxide

KW - Smooth

KW - Ultraviolet rays

KW - Vascular

UR - http://www.scopus.com/inward/record.url?scp=0028214039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028214039&partnerID=8YFLogxK

U2 - 10.1161/01.HYP.23.6.894

DO - 10.1161/01.HYP.23.6.894

M3 - Article

VL - 23

SP - 894

EP - 898

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 6

ER -