A point mutation in the ethylene-inducing xylanase elicitor inhibits the β-1-4-endoxylanase activity but not the elicitation activity

Noa Furman-Matarasso, Eitan Cohen, Quansheng Du, Nor Chejanovsky, Uri Hanania, Adi Avni

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Abstract

Ethylene-inducing xylanase (EIX) elicits plant defense responses in certain tobacco (Nicotiana tabacum) and tomato cultivars in addition to its xylan degradation activity. It is not clear, however, whether elicitation occurs by cell wall fragments released by the enzymatic activity or by the xylanase protein interacting directly with the plant cells. We cloned the gene encoding EIX protein and overexpressed it in insect cells. To determine the relationship between the two activities, substitution of amino acids in the xylanase active site was performed. Substitution at glutamic acid-86 or -177 with glutamine (Gln), aspartic acid (Asp), or glycine (Gly) inhibited the β-1-4-endoxylanase activity. Mutants having Asp-86 or Gln-177 also lost the ability to induce the hypersensitive response and ethylene biosynthesis. However, mutants having Gln-86, Gly-86, Asp-177, or Gly-177 retained ability to induce ethylene biosynthesis and the hypersensitive response. Our data show that the xylanase activity of EIX elicitor can be separated from the elicitation process, as some of the mutants lack the former but retain the latter.

Original languageEnglish (US)
Pages (from-to)345-351
Number of pages7
JournalPlant Physiology
Volume121
Issue number2
StatePublished - Oct 1 1999

Fingerprint

endo-1,4-beta-xylanase
Endo-1,4-beta Xylanases
xylanases
point mutation
Point Mutation
ethylene
Glutamine
Aspartic Acid
Glycine
aspartic acid
glycine (amino acid)
glutamine
Tobacco
hypersensitive response
ethylene production
mutants
Xylans
Plant Cells
Amino Acid Substitution
Lycopersicon esculentum

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

A point mutation in the ethylene-inducing xylanase elicitor inhibits the β-1-4-endoxylanase activity but not the elicitation activity. / Furman-Matarasso, Noa; Cohen, Eitan; Du, Quansheng; Chejanovsky, Nor; Hanania, Uri; Avni, Adi.

In: Plant Physiology, Vol. 121, No. 2, 01.10.1999, p. 345-351.

Research output: Contribution to journalArticle

Furman-Matarasso, N, Cohen, E, Du, Q, Chejanovsky, N, Hanania, U & Avni, A 1999, 'A point mutation in the ethylene-inducing xylanase elicitor inhibits the β-1-4-endoxylanase activity but not the elicitation activity', Plant Physiology, vol. 121, no. 2, pp. 345-351.
Furman-Matarasso, Noa ; Cohen, Eitan ; Du, Quansheng ; Chejanovsky, Nor ; Hanania, Uri ; Avni, Adi. / A point mutation in the ethylene-inducing xylanase elicitor inhibits the β-1-4-endoxylanase activity but not the elicitation activity. In: Plant Physiology. 1999 ; Vol. 121, No. 2. pp. 345-351.
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