Abstract
A method is described for the rapid, accurate measurement of single cells in situ using microfluorimetry. This method involves a shutter system, which allows irradiation of single cells for fractions of a second and a peak fluorescence intensity recording device. In this way errors due to fluorochrome fading are almost eliminated and standard deviations of less than 5% are obtained. Hoechst 33258 has been used as a quantitative fluorochrome. Optimum fixation and staining conditions on glass and plastic tissue culture vessels are described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 206-210 |
| Number of pages | 5 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 28 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1980 |
| Externally published | Yes |
ASJC Scopus subject areas
- Anatomy
- Histology