A requirement for the Rho-family GTP exchange factor Vav in positive and negative selection of thymocytes

Martin Turner, P. Joseph Mee, Alice E. Walters, Marian E. Quinn, Andrew L. Mellor, Rose Zamoyska, Victor L J Tybulewicz

Research output: Contribution to journalArticle

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Abstract

The T cell repertoire is shaped by positive and negative selection of thymocytes that express low levels of T cell receptor (TCR) and both CD4 and CD8. TCR-mediated signals that determine these selection processes are only partly understood. Vav, a GDP-GTP exchange factor for Rho-family proteins, is tyrosine phosphorylated following TCR stimulation, suggesting that it may transduce TCR signals. We now demonstrate that mice lacking Vav are viable and display a profound defect in the positive selection of both class I- and class II-restricted T cells. In contrast, Vav is not essential far negative selection, though in its absence negative selection is much less effective. Vav may influence the efficiency of TCR-induced selection events by regulating the intracellular calcium flux of thymocytes.

Original languageEnglish (US)
Pages (from-to)451-460
Number of pages10
JournalImmunity
Volume7
Issue number4
DOIs
StatePublished - Jan 1 1997

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Thymocytes
T-Cell Antigen Receptor
Guanosine Triphosphate
T-Lymphocytes
Guanine Nucleotide Exchange Factors
Tyrosine
Calcium
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Cite this

Turner, M., Mee, P. J., Walters, A. E., Quinn, M. E., Mellor, A. L., Zamoyska, R., & Tybulewicz, V. L. J. (1997). A requirement for the Rho-family GTP exchange factor Vav in positive and negative selection of thymocytes. Immunity, 7(4), 451-460. https://doi.org/10.1016/S1074-7613(00)80367-2

A requirement for the Rho-family GTP exchange factor Vav in positive and negative selection of thymocytes. / Turner, Martin; Mee, P. Joseph; Walters, Alice E.; Quinn, Marian E.; Mellor, Andrew L.; Zamoyska, Rose; Tybulewicz, Victor L J.

In: Immunity, Vol. 7, No. 4, 01.01.1997, p. 451-460.

Research output: Contribution to journalArticle

Turner, M, Mee, PJ, Walters, AE, Quinn, ME, Mellor, AL, Zamoyska, R & Tybulewicz, VLJ 1997, 'A requirement for the Rho-family GTP exchange factor Vav in positive and negative selection of thymocytes', Immunity, vol. 7, no. 4, pp. 451-460. https://doi.org/10.1016/S1074-7613(00)80367-2
Turner, Martin ; Mee, P. Joseph ; Walters, Alice E. ; Quinn, Marian E. ; Mellor, Andrew L. ; Zamoyska, Rose ; Tybulewicz, Victor L J. / A requirement for the Rho-family GTP exchange factor Vav in positive and negative selection of thymocytes. In: Immunity. 1997 ; Vol. 7, No. 4. pp. 451-460.
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