A retroviral vector expressing human interferon gamma upregulates MHC antigen expression in human breast cancer and leukemia cell lines.

K Cornetta, D Berebitsky, M Behnia, C Traycoff, E F Srour, G W Sledge

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The use of cytokines to modify antigen expression on syngeneic murine tumor cells has led to immunization of the host from subsequent challenges with the parent tumor cell line. To determine whether this approach can be applied to human malignancies we introduced the human interferon gamma gene (IFN gamma) into the human breast cancer cell lines MDA-MB-435 and MDA-MB-231 using retroviral-mediated gene transfer. Retroviral transfer of the IFN gamma gene was associated with decreased growth, decreased tumor invasiveness, IFN gamma production, and upregulation of MHC antigens. While the MDA-MB-435 produced higher levels of IFN gamma than MDA-MB-231, MHC class I and class II antigens were upregulated in both cell lines. Introduction of this vector into the human leukemia cell line K562 led to increased expression of MHC class I antigens, but not class II. Our findings suggest that expression of interferon gamma in breast cancer cells may lead to increased recognition of breast cancer cell
Original languageUndefined
Pages (from-to)91 - 98
JournalCancer Gene Therapy
Volume1
Issue number2
StatePublished - 1994

Keywords

  • Gene Expression Regulation, Neoplastic*, Antigens, Neoplasm/*biosynthesis, Breast Neoplasms/*pathology, HLA Antigens/*biosynthesis, Interferon-gamma/*genetics, Leukemia, Erythroblastic, Acute/*pathology, Antigens, Neoplasm/genetics, Breast Neoplasms/immunology, Breast Neoplasms/metabolism, Collagen, Drug Combinations, Gene Expression Regulation, Leukemic, Genetic Therapy, Genetic Vectors, HLA Antigens/genetics, Humans, Immunotherapy, Interferon-gamma/biosynthesis, Interferon-gamma/physiology, Laminin, Leukemia, Erythroblastic, Acute/immunology, Leukemia, Erythroblastic, Acute/metabolism, Neoplasm Invasiveness, Proteoglycans, Recombinant Proteins, Retroviridae, Transfection, Tumor Cells, Cultured

Cite this

A retroviral vector expressing human interferon gamma upregulates MHC antigen expression in human breast cancer and leukemia cell lines. / Cornetta, K; Berebitsky, D; Behnia, M; Traycoff, C; Srour, E F; Sledge, G W.

In: Cancer Gene Therapy, Vol. 1, No. 2, 1994, p. 91 - 98.

Research output: Contribution to journalArticle

Cornetta, K ; Berebitsky, D ; Behnia, M ; Traycoff, C ; Srour, E F ; Sledge, G W. / A retroviral vector expressing human interferon gamma upregulates MHC antigen expression in human breast cancer and leukemia cell lines. In: Cancer Gene Therapy. 1994 ; Vol. 1, No. 2. pp. 91 - 98.
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abstract = "The use of cytokines to modify antigen expression on syngeneic murine tumor cells has led to immunization of the host from subsequent challenges with the parent tumor cell line. To determine whether this approach can be applied to human malignancies we introduced the human interferon gamma gene (IFN gamma) into the human breast cancer cell lines MDA-MB-435 and MDA-MB-231 using retroviral-mediated gene transfer. Retroviral transfer of the IFN gamma gene was associated with decreased growth, decreased tumor invasiveness, IFN gamma production, and upregulation of MHC antigens. While the MDA-MB-435 produced higher levels of IFN gamma than MDA-MB-231, MHC class I and class II antigens were upregulated in both cell lines. Introduction of this vector into the human leukemia cell line K562 led to increased expression of MHC class I antigens, but not class II. Our findings suggest that expression of interferon gamma in breast cancer cells may lead to increased recognition of breast cancer cell",
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AU - Srour, E F

AU - Sledge, G W

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AB - The use of cytokines to modify antigen expression on syngeneic murine tumor cells has led to immunization of the host from subsequent challenges with the parent tumor cell line. To determine whether this approach can be applied to human malignancies we introduced the human interferon gamma gene (IFN gamma) into the human breast cancer cell lines MDA-MB-435 and MDA-MB-231 using retroviral-mediated gene transfer. Retroviral transfer of the IFN gamma gene was associated with decreased growth, decreased tumor invasiveness, IFN gamma production, and upregulation of MHC antigens. While the MDA-MB-435 produced higher levels of IFN gamma than MDA-MB-231, MHC class I and class II antigens were upregulated in both cell lines. Introduction of this vector into the human leukemia cell line K562 led to increased expression of MHC class I antigens, but not class II. Our findings suggest that expression of interferon gamma in breast cancer cells may lead to increased recognition of breast cancer cell

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