TY - JOUR
T1 - A robust, inexpensive filter for blocking UVC radiation in broad-spectrum 'UVB' lamps
AU - Lepre, A. M.
AU - Sutherland, J. C.
AU - Trunk, J. G.
AU - Sutherland, B. M.
N1 - Funding Information:
This research was supported by the Oftice of Biological and Environmental Research of the LS Department of Energy, and by Cooperative Research and Development Agreement No. BNL-C-95-04. AML was the recipient of ;I fellowship from the Science and Engineering program. Brookhaven National Laboratory. We wish to thank Paula Bennett for instruction in DNA isolation, William and Martha LaMar for their gracious hospitality during Ihe writing ofthi\ manuscript and Dr Richard Setlow and offcr-
PY - 1998/4
Y1 - 1998/4
N2 - Accurate studies of the biological effects of UVB radiation require suitable laboratory sources. Lamps labeled as UVB sources often emit UVC radiation that contributes significantly to the levels of DNA damage. The UVC from an unfiltered UVB source produced more pyrimidine dimers in soybean DNA than a lamp filtered by a Pyrex dish that removes wavelengths of < 280 nm. Calculations based on action spectra and on the emission spectra of unfiltered lamps indicate that UVC contributes ~ 13%, 4% and ~ 1% of the total dimers induced in unshielded cells or DNA, alfalfa cotyledons, and human skin, respectively. Further, relative to a Pyrex dish-filtered lamp, an unfiltered lamp would produce ~ 7-, 2.4- or 2.8-fold more dimers in these three biological systems. We report here that a Pyrex dish provides an effective, stable, robust and inexpensive filter for reducing or excluding the contribution of UVC to damage induced by broad-spectrum 'UVB' lamps.
AB - Accurate studies of the biological effects of UVB radiation require suitable laboratory sources. Lamps labeled as UVB sources often emit UVC radiation that contributes significantly to the levels of DNA damage. The UVC from an unfiltered UVB source produced more pyrimidine dimers in soybean DNA than a lamp filtered by a Pyrex dish that removes wavelengths of < 280 nm. Calculations based on action spectra and on the emission spectra of unfiltered lamps indicate that UVC contributes ~ 13%, 4% and ~ 1% of the total dimers induced in unshielded cells or DNA, alfalfa cotyledons, and human skin, respectively. Further, relative to a Pyrex dish-filtered lamp, an unfiltered lamp would produce ~ 7-, 2.4- or 2.8-fold more dimers in these three biological systems. We report here that a Pyrex dish provides an effective, stable, robust and inexpensive filter for reducing or excluding the contribution of UVC to damage induced by broad-spectrum 'UVB' lamps.
KW - Cyclobutyl pyrimidine dimer
KW - Environmental photobiology
KW - UV filter
KW - UV radiation
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U2 - 10.1016/S1011-1344(98)00079-7
DO - 10.1016/S1011-1344(98)00079-7
M3 - Article
C2 - 9639912
AN - SCOPUS:0032052033
VL - 43
SP - 34
EP - 40
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
IS - 1
ER -