A side by side comparison of cytology and biomarkers for bladder cancer detection

Grethchen L. Schroeder, Maria Fernanda Lorenzo-Gomez, Stefan H. Hautmann, Martin G. Friedrich, Sinan Ekici, Hartwig Huland, Vinata Lokeshwar

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Purpose: The identification of accurate bladder tumor markers/tests could improve diagnosis, recurrence monitoring and treatment in patients with bladder cancer. In this study we compared the efficacy of the hyaluronic acid (HA)-hyaluronidase (HAase), BTA-Stat (Bard/Bion Diagnostics, Redmond, Washington), Hemastix (Bayer Corp., Elkhart, Indiana) (hematuria detection) and UBC-Rapid (IDL Biotech, Borlänger, Sweden) tests, and cytology to detect bladder cancer. The HA-HAase test measures urinary HA and HAase levels, BTA-Stat detects complement factor-H and H related protein in urine, the Hemastix hemoglobin dipstick detects hematuria and UBC-Rapid detects cytokeratin 8 and 18 fragments in urine. Materials and Methods: A total of 138 urine specimens from 115 patients were collected at University Hospital Hamburg-Eppendorf, including 59 with active bladder cancer and 79 with a history of bladder cancer (73) or with benign genitourinary conditions (6). Specimens were assayed by the HA-HAase test, BTA-Stat, Hemastix (hemoglobin dipstick) and UBC-Rapid. Cystoscopy and histological findings were used to make the clinical diagnosis. Cytology results were available on 92 patients. Results: In a side by side comparison the HA-HAase test, cytology, BTA-Stat, Hemastix and UBC-Rapid had 88.1%, 70.6%, 52.5%, 50.8% and 35.6% sensitivity, and 81%, 81%, 76.7%, 78.2% and 75% specificity, respectively. The accuracy, and negative and positive predictive values of the HA-HAase test were the highest (84.1%, 90.1% and 77.6%), followed by cytology (77.2%, 82.5% and 68.6%), Hemastix (66.4%, 67.8% and 63.8%), BTA-Stat (66.2%, 67.8% and 63.3%) and UBC-Rapid (57.8%, 60% and 52.5%), respectively. Conclusions: The HA-HAase test is superior to cytology, BTA-Stat, Hemastix and UBC-Rapid for detecting bladder cancer recurrence. A side-by-side comparison of tumor markers should help identify a marker for monitoring bladder cancer recurrence.

Original languageEnglish (US)
Pages (from-to)1123-1126
Number of pages4
JournalJournal of Urology
Volume172
Issue number3
DOIs
StatePublished - Sep 2004

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Hyaluronoglucosaminidase
Hyaluronic Acid
Urinary Bladder Neoplasms
Cell Biology
Biomarkers
Urine
Hematuria
Tumor Biomarkers
Recurrence
Hemoglobins
Keratin-8
Keratin-18
Complement Factor H
Cystoscopy
Sweden

Keywords

  • Bladder
  • Bladder neoplasms
  • Cytology
  • Hematuria
  • Tumor markers, biological

ASJC Scopus subject areas

  • Urology

Cite this

Schroeder, G. L., Lorenzo-Gomez, M. F., Hautmann, S. H., Friedrich, M. G., Ekici, S., Huland, H., & Lokeshwar, V. (2004). A side by side comparison of cytology and biomarkers for bladder cancer detection. Journal of Urology, 172(3), 1123-1126. https://doi.org/10.1097/01.ju.0000134347.14643.ab

A side by side comparison of cytology and biomarkers for bladder cancer detection. / Schroeder, Grethchen L.; Lorenzo-Gomez, Maria Fernanda; Hautmann, Stefan H.; Friedrich, Martin G.; Ekici, Sinan; Huland, Hartwig; Lokeshwar, Vinata.

In: Journal of Urology, Vol. 172, No. 3, 09.2004, p. 1123-1126.

Research output: Contribution to journalArticle

Schroeder, GL, Lorenzo-Gomez, MF, Hautmann, SH, Friedrich, MG, Ekici, S, Huland, H & Lokeshwar, V 2004, 'A side by side comparison of cytology and biomarkers for bladder cancer detection', Journal of Urology, vol. 172, no. 3, pp. 1123-1126. https://doi.org/10.1097/01.ju.0000134347.14643.ab
Schroeder GL, Lorenzo-Gomez MF, Hautmann SH, Friedrich MG, Ekici S, Huland H et al. A side by side comparison of cytology and biomarkers for bladder cancer detection. Journal of Urology. 2004 Sep;172(3):1123-1126. https://doi.org/10.1097/01.ju.0000134347.14643.ab
Schroeder, Grethchen L. ; Lorenzo-Gomez, Maria Fernanda ; Hautmann, Stefan H. ; Friedrich, Martin G. ; Ekici, Sinan ; Huland, Hartwig ; Lokeshwar, Vinata. / A side by side comparison of cytology and biomarkers for bladder cancer detection. In: Journal of Urology. 2004 ; Vol. 172, No. 3. pp. 1123-1126.
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abstract = "Purpose: The identification of accurate bladder tumor markers/tests could improve diagnosis, recurrence monitoring and treatment in patients with bladder cancer. In this study we compared the efficacy of the hyaluronic acid (HA)-hyaluronidase (HAase), BTA-Stat (Bard/Bion Diagnostics, Redmond, Washington), Hemastix (Bayer Corp., Elkhart, Indiana) (hematuria detection) and UBC-Rapid (IDL Biotech, Borl{\"a}nger, Sweden) tests, and cytology to detect bladder cancer. The HA-HAase test measures urinary HA and HAase levels, BTA-Stat detects complement factor-H and H related protein in urine, the Hemastix hemoglobin dipstick detects hematuria and UBC-Rapid detects cytokeratin 8 and 18 fragments in urine. Materials and Methods: A total of 138 urine specimens from 115 patients were collected at University Hospital Hamburg-Eppendorf, including 59 with active bladder cancer and 79 with a history of bladder cancer (73) or with benign genitourinary conditions (6). Specimens were assayed by the HA-HAase test, BTA-Stat, Hemastix (hemoglobin dipstick) and UBC-Rapid. Cystoscopy and histological findings were used to make the clinical diagnosis. Cytology results were available on 92 patients. Results: In a side by side comparison the HA-HAase test, cytology, BTA-Stat, Hemastix and UBC-Rapid had 88.1{\%}, 70.6{\%}, 52.5{\%}, 50.8{\%} and 35.6{\%} sensitivity, and 81{\%}, 81{\%}, 76.7{\%}, 78.2{\%} and 75{\%} specificity, respectively. The accuracy, and negative and positive predictive values of the HA-HAase test were the highest (84.1{\%}, 90.1{\%} and 77.6{\%}), followed by cytology (77.2{\%}, 82.5{\%} and 68.6{\%}), Hemastix (66.4{\%}, 67.8{\%} and 63.8{\%}), BTA-Stat (66.2{\%}, 67.8{\%} and 63.3{\%}) and UBC-Rapid (57.8{\%}, 60{\%} and 52.5{\%}), respectively. Conclusions: The HA-HAase test is superior to cytology, BTA-Stat, Hemastix and UBC-Rapid for detecting bladder cancer recurrence. A side-by-side comparison of tumor markers should help identify a marker for monitoring bladder cancer recurrence.",
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N2 - Purpose: The identification of accurate bladder tumor markers/tests could improve diagnosis, recurrence monitoring and treatment in patients with bladder cancer. In this study we compared the efficacy of the hyaluronic acid (HA)-hyaluronidase (HAase), BTA-Stat (Bard/Bion Diagnostics, Redmond, Washington), Hemastix (Bayer Corp., Elkhart, Indiana) (hematuria detection) and UBC-Rapid (IDL Biotech, Borlänger, Sweden) tests, and cytology to detect bladder cancer. The HA-HAase test measures urinary HA and HAase levels, BTA-Stat detects complement factor-H and H related protein in urine, the Hemastix hemoglobin dipstick detects hematuria and UBC-Rapid detects cytokeratin 8 and 18 fragments in urine. Materials and Methods: A total of 138 urine specimens from 115 patients were collected at University Hospital Hamburg-Eppendorf, including 59 with active bladder cancer and 79 with a history of bladder cancer (73) or with benign genitourinary conditions (6). Specimens were assayed by the HA-HAase test, BTA-Stat, Hemastix (hemoglobin dipstick) and UBC-Rapid. Cystoscopy and histological findings were used to make the clinical diagnosis. Cytology results were available on 92 patients. Results: In a side by side comparison the HA-HAase test, cytology, BTA-Stat, Hemastix and UBC-Rapid had 88.1%, 70.6%, 52.5%, 50.8% and 35.6% sensitivity, and 81%, 81%, 76.7%, 78.2% and 75% specificity, respectively. The accuracy, and negative and positive predictive values of the HA-HAase test were the highest (84.1%, 90.1% and 77.6%), followed by cytology (77.2%, 82.5% and 68.6%), Hemastix (66.4%, 67.8% and 63.8%), BTA-Stat (66.2%, 67.8% and 63.3%) and UBC-Rapid (57.8%, 60% and 52.5%), respectively. Conclusions: The HA-HAase test is superior to cytology, BTA-Stat, Hemastix and UBC-Rapid for detecting bladder cancer recurrence. A side-by-side comparison of tumor markers should help identify a marker for monitoring bladder cancer recurrence.

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