A simple and selective kinetic spectrophotometric method for the determination of kanamycin using acetylacetone-formaldehyde reagent in N,N'-dimethylformamide medium

A. S. Ahmad, MD Nasrul Hoda, M. Ahmad, F. Islam, S. Z. Qureshi

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Abstract

A simple, sensitive, and selective kinetic spectrophotometric method for the determination of kanamycin in pure form and pharmaceutical formulations is described. The method is based on the measurement of the intensity of the yellow chromogen formed by the reaction between kanamycin and acetylacetone- formaldehyde reagent in a N,N'-dimethyl formamide medium. The variable-time method was used to evaluate the rate of reaction of the colored chromogen formed at 410 nm. The reaction conditions were optimized and the calibration graph was found to be linear in the range 60-160 μg/mL. The results obtained by the developed and reference methods are in good agreement. Various statistical parameters were evaluated to establish the precision, accuracy, repeatability, and reproducibility of the proposed method.

Original languageEnglish (US)
Pages (from-to)870-874
Number of pages5
JournalJournal of Analytical Chemistry
Volume61
Issue number9
DOIs
StatePublished - Sep 1 2006

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ASJC Scopus subject areas

  • Analytical Chemistry

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