A simple two-step purification of human and monkey alpha fetoprotein from amniotic fluid and serum.

J. A. Leal, Brooks Allen Keel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We developed a two-step purification system to characterize alpha fetoprotein (AFP) in early gestation amniotic fluid and late gestation fetal serum or cord blood from monkey and human. It involves only two chromatographic steps, allows preparative purification using up to 12 ml of starting sample, can purify up to 350 micrograms of AFP at one time, and can be used to purify both fetal serum or amniotic fluid AFP from two different species. This procedure will allow detailed biochemical analysis of purified AFP from different stages of fetal development.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalJournal of Medical Primatology
Volume20
Issue number1
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

amniotic fluid
alpha-Fetoproteins
Amniotic Fluid
blood serum
Haplorhini
monkeys
pregnancy
fetal development
Serum
Fetal Blood
Pregnancy
blood
Fetal Development
sampling
human AFP protein

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

A simple two-step purification of human and monkey alpha fetoprotein from amniotic fluid and serum. / Leal, J. A.; Keel, Brooks Allen.

In: Journal of Medical Primatology, Vol. 20, No. 1, 01.01.1991, p. 35-41.

Research output: Contribution to journalArticle

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