A simple two-step purification of human and monkey alpha fetoprotein from amniotic fluid and serum.

J. A. Leal, B. A. Keel

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We developed a two-step purification system to characterize alpha fetoprotein (AFP) in early gestation amniotic fluid and late gestation fetal serum or cord blood from monkey and human. It involves only two chromatographic steps, allows preparative purification using up to 12 ml of starting sample, can purify up to 350 micrograms of AFP at one time, and can be used to purify both fetal serum or amniotic fluid AFP from two different species. This procedure will allow detailed biochemical analysis of purified AFP from different stages of fetal development.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalJournal of medical primatology
Volume20
Issue number1
StatePublished - 1991

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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