A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells

Shuang Huang, Vijay Reddy, Nabarun Dasgupta, Glen R. Nemerow

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A 46-kDa receptor, coxsackievirus-adenovirus (Ad) receptor (CAR), mediates cell attachment of a number of different Ad serotypes; however, not all Ad serotypes utilize this receptor for infection. Moreover, the precise amino acid sequences in the Ad fiber protein that mediate cell attachment have yet to be identified. We investigated the interaction of subgroup D Ads with human ocular cells. Ad serotype 37 (Ad37), a virus associated with epidemic keratoconjunctivitis, but not a closely related virus serotype, Ad19p, exhibited preferential binding to and infection of human conjunctival cells. A single amino acid substitution in the Ad19p fiber distal domain (knob), Glu(240) to Lys, conferred binding to conjunctival cells, while the reverse substitution in the Ad37 fiber abrogated cell binding. These findings provide new information on the fiber sequences that regulate Ad host cell tropism.

Original languageEnglish (US)
Pages (from-to)2798-2802
Number of pages5
JournalJournal of Virology
Volume73
Issue number4
StatePublished - Apr 1 1999

Fingerprint

Adenoviridae
serotypes
Amino Acids
amino acids
cells
receptors
Viral Tropism
Keratoconjunctivitis
Viruses
fiber cells
keratoconjunctivitis
viruses
Enterovirus
tropisms
amino acid substitution
Amino Acid Substitution
Infection
infection
Amino Acid Sequence
amino acid sequences

ASJC Scopus subject areas

  • Immunology

Cite this

Huang, S., Reddy, V., Dasgupta, N., & Nemerow, G. R. (1999). A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells. Journal of Virology, 73(4), 2798-2802.

A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells. / Huang, Shuang; Reddy, Vijay; Dasgupta, Nabarun; Nemerow, Glen R.

In: Journal of Virology, Vol. 73, No. 4, 01.04.1999, p. 2798-2802.

Research output: Contribution to journalArticle

Huang, S, Reddy, V, Dasgupta, N & Nemerow, GR 1999, 'A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells', Journal of Virology, vol. 73, no. 4, pp. 2798-2802.
Huang, Shuang ; Reddy, Vijay ; Dasgupta, Nabarun ; Nemerow, Glen R. / A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells. In: Journal of Virology. 1999 ; Vol. 73, No. 4. pp. 2798-2802.
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