A single amino acid in the adenovirus type 37 fiber confers binding to human conjunctival cells

Shuang Huang, Vijay Reddy, Nabarun Dasgupta, Glen R. Nemerow

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

A 46-kDa receptor, coxsackievirus-adenovirus (Ad) receptor (CAR), mediates cell attachment of a number of different Ad serotypes; however, not all Ad serotypes utilize this receptor for infection. Moreover, the precise amino acid sequences in the Ad fiber protein that mediate cell attachment have yet to be identified. We investigated the interaction of subgroup D Ads with human ocular cells. Ad serotype 37 (Ad37), a virus associated with epidemic keratoconjunctivitis, but not a closely related virus serotype, Ad19p, exhibited preferential binding to and infection of human conjunctival cells. A single amino acid substitution in the Ad19p fiber distal domain (knob), Glu(240) to Lys, conferred binding to conjunctival cells, while the reverse substitution in the Ad37 fiber abrogated cell binding. These findings provide new information on the fiber sequences that regulate Ad host cell tropism.

Original languageEnglish (US)
Pages (from-to)2798-2802
Number of pages5
JournalJournal of Virology
Volume73
Issue number4
DOIs
StatePublished - Apr 1999

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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