A single high-fat meal provokes pathological erythrocyte remodeling and increases myeloperoxidase levels: implications for acute coronary syndrome

Tyler W. Benson, Neal Lee Weintraub, Ha Won Kim, Nichole Seigler, Sanjiv Kumar, Jonathan Pye, Tetsuo Horimatsu, Rod Pellenberg, David W Stepp, Rudolf Lucas, Vladimir Y. Bogdanov, Sheldon E. Litwin, Julia Elizabeth Brittain, Ryan A. Harris

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

High-fat meal (HFM) consumption can produce acute lipemia and trigger myocardial infarction in patients with atherosclerosis, but the mechanisms are poorly understood. Erythrocytes (red blood cells, RBCs) intimately interact with inflammatory cells and blood vessels and play a complex role in regulating vascular function. Chronic high-fat feeding in mice induces pathological RBC remodeling, suggesting a novel link between HFM, RBCs, and vascular dysfunction. However, whether acute HFM can induce RBC remodeling in humans is unknown. Ten healthy individuals were subjected to biochemical testing and assessment of endothelial-dependent flow-mediated dilation (FMD) before and after a single HFM or iso-caloric meal (ICM). Following the HFM, triglyceride, cholesterol, and free fatty acid levels were all significantly increased, in conjunction with impaired post-prandial FMD. Additionally, peripheral blood smears demonstrated microcytes, remodeled RBCs, and fatty monocytes. Increased intracellular ROS and nitration of protein band 3 was detected in RBCs following the HFM. The HFM elevated plasma and RBC-bound myeloperoxidase (MPO), which was associated with impaired FMD and oxidation of HDL. Monocytic cells exposed to lipid in vitro released MPO, while porcine coronary arteries exposed to fatty acids ex vivo took up MPO. We demonstrate in humans that a single HFM induces pathological RBC remodeling and concurrently elevates MPO, which can potentially enter the blood vessel wall to trigger oxidative stress and destabilize vulnerable plaques. These novel findings may have implications for the short-term risk of HFM consumption and alimentary lipemia in patients with atherosclerosis.

Original languageEnglish (US)
Pages (from-to)1300-1310
Number of pages11
JournalLaboratory Investigation
Volume98
Issue number10
DOIs
StatePublished - Oct 1 2018

Fingerprint

Acute Coronary Syndrome
Peroxidase
Meals
Erythrocytes
Fats
Blood Vessels
Dilatation
Hyperlipidemias
Atherosclerosis
Erythrocyte Anion Exchange Protein 1
Nonesterified Fatty Acids
Monocytes
Coronary Vessels
Triglycerides
Oxidative Stress
Swine
Fatty Acids
Myocardial Infarction
Cholesterol
Lipids

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

A single high-fat meal provokes pathological erythrocyte remodeling and increases myeloperoxidase levels : implications for acute coronary syndrome. / Benson, Tyler W.; Weintraub, Neal Lee; Kim, Ha Won; Seigler, Nichole; Kumar, Sanjiv; Pye, Jonathan; Horimatsu, Tetsuo; Pellenberg, Rod; Stepp, David W; Lucas, Rudolf; Bogdanov, Vladimir Y.; Litwin, Sheldon E.; Brittain, Julia Elizabeth; Harris, Ryan A.

In: Laboratory Investigation, Vol. 98, No. 10, 01.10.2018, p. 1300-1310.

Research output: Contribution to journalArticle

Benson, Tyler W. ; Weintraub, Neal Lee ; Kim, Ha Won ; Seigler, Nichole ; Kumar, Sanjiv ; Pye, Jonathan ; Horimatsu, Tetsuo ; Pellenberg, Rod ; Stepp, David W ; Lucas, Rudolf ; Bogdanov, Vladimir Y. ; Litwin, Sheldon E. ; Brittain, Julia Elizabeth ; Harris, Ryan A. / A single high-fat meal provokes pathological erythrocyte remodeling and increases myeloperoxidase levels : implications for acute coronary syndrome. In: Laboratory Investigation. 2018 ; Vol. 98, No. 10. pp. 1300-1310.
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T1 - A single high-fat meal provokes pathological erythrocyte remodeling and increases myeloperoxidase levels

T2 - implications for acute coronary syndrome

AU - Benson, Tyler W.

AU - Weintraub, Neal Lee

AU - Kim, Ha Won

AU - Seigler, Nichole

AU - Kumar, Sanjiv

AU - Pye, Jonathan

AU - Horimatsu, Tetsuo

AU - Pellenberg, Rod

AU - Stepp, David W

AU - Lucas, Rudolf

AU - Bogdanov, Vladimir Y.

AU - Litwin, Sheldon E.

AU - Brittain, Julia Elizabeth

AU - Harris, Ryan A.

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N2 - High-fat meal (HFM) consumption can produce acute lipemia and trigger myocardial infarction in patients with atherosclerosis, but the mechanisms are poorly understood. Erythrocytes (red blood cells, RBCs) intimately interact with inflammatory cells and blood vessels and play a complex role in regulating vascular function. Chronic high-fat feeding in mice induces pathological RBC remodeling, suggesting a novel link between HFM, RBCs, and vascular dysfunction. However, whether acute HFM can induce RBC remodeling in humans is unknown. Ten healthy individuals were subjected to biochemical testing and assessment of endothelial-dependent flow-mediated dilation (FMD) before and after a single HFM or iso-caloric meal (ICM). Following the HFM, triglyceride, cholesterol, and free fatty acid levels were all significantly increased, in conjunction with impaired post-prandial FMD. Additionally, peripheral blood smears demonstrated microcytes, remodeled RBCs, and fatty monocytes. Increased intracellular ROS and nitration of protein band 3 was detected in RBCs following the HFM. The HFM elevated plasma and RBC-bound myeloperoxidase (MPO), which was associated with impaired FMD and oxidation of HDL. Monocytic cells exposed to lipid in vitro released MPO, while porcine coronary arteries exposed to fatty acids ex vivo took up MPO. We demonstrate in humans that a single HFM induces pathological RBC remodeling and concurrently elevates MPO, which can potentially enter the blood vessel wall to trigger oxidative stress and destabilize vulnerable plaques. These novel findings may have implications for the short-term risk of HFM consumption and alimentary lipemia in patients with atherosclerosis.

AB - High-fat meal (HFM) consumption can produce acute lipemia and trigger myocardial infarction in patients with atherosclerosis, but the mechanisms are poorly understood. Erythrocytes (red blood cells, RBCs) intimately interact with inflammatory cells and blood vessels and play a complex role in regulating vascular function. Chronic high-fat feeding in mice induces pathological RBC remodeling, suggesting a novel link between HFM, RBCs, and vascular dysfunction. However, whether acute HFM can induce RBC remodeling in humans is unknown. Ten healthy individuals were subjected to biochemical testing and assessment of endothelial-dependent flow-mediated dilation (FMD) before and after a single HFM or iso-caloric meal (ICM). Following the HFM, triglyceride, cholesterol, and free fatty acid levels were all significantly increased, in conjunction with impaired post-prandial FMD. Additionally, peripheral blood smears demonstrated microcytes, remodeled RBCs, and fatty monocytes. Increased intracellular ROS and nitration of protein band 3 was detected in RBCs following the HFM. The HFM elevated plasma and RBC-bound myeloperoxidase (MPO), which was associated with impaired FMD and oxidation of HDL. Monocytic cells exposed to lipid in vitro released MPO, while porcine coronary arteries exposed to fatty acids ex vivo took up MPO. We demonstrate in humans that a single HFM induces pathological RBC remodeling and concurrently elevates MPO, which can potentially enter the blood vessel wall to trigger oxidative stress and destabilize vulnerable plaques. These novel findings may have implications for the short-term risk of HFM consumption and alimentary lipemia in patients with atherosclerosis.

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