A Single Mammalian Mitochondrial Translation Initiation Factor Functionally Replaces Two Bacterial Factors

Rahul Gaur, Domenick Grasso, Partha P. Datta, P. D.V. Krishna, Gautam Das, Angela C Spencer, Rajendra K. Agrawal, Linda Spremulli, Umesh Varshney

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The mechanism of translation in eubacteria and organelles is thought to be similar. In eubacteria, the three initiation factors IF1, IF2, and IF3 are vital. Although the homologs of IF2 and IF3 are found in mammalian mitochondria, an IF1 homolog has never been detected. Here, we show that bovine mitochondrial IF2 (IF2mt) complements E. coli containing a deletion of the IF2 gene (E. coli ΔinfB). We find that IF1 is no longer essential in an IF2mt-supported E. coli ΔinfB strain. Furthermore, biochemical and molecular modeling data show that a conserved insertion of 37 amino acids in the IF2mt substitutes for the function of IF1. Deletion of this insertion from IF2mt supports E. coli for the essential function of IF2. However, in this background, IF1 remains essential. These observations provide strong evidence that a single factor (IF2mt) in mammalian mitochondria performs the functions of two eubacterial factors, IF1 and IF2.

Original languageEnglish (US)
Pages (from-to)180-190
Number of pages11
JournalMolecular Cell
Volume29
Issue number2
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

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Peptide Initiation Factors
Escherichia coli
Mitochondria
Bacteria
Gene Deletion
Organelles
Amino Acids

Keywords

  • DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

A Single Mammalian Mitochondrial Translation Initiation Factor Functionally Replaces Two Bacterial Factors. / Gaur, Rahul; Grasso, Domenick; Datta, Partha P.; Krishna, P. D.V.; Das, Gautam; Spencer, Angela C; Agrawal, Rajendra K.; Spremulli, Linda; Varshney, Umesh.

In: Molecular Cell, Vol. 29, No. 2, 01.02.2008, p. 180-190.

Research output: Contribution to journalArticle

Gaur, R, Grasso, D, Datta, PP, Krishna, PDV, Das, G, Spencer, AC, Agrawal, RK, Spremulli, L & Varshney, U 2008, 'A Single Mammalian Mitochondrial Translation Initiation Factor Functionally Replaces Two Bacterial Factors', Molecular Cell, vol. 29, no. 2, pp. 180-190. https://doi.org/10.1016/j.molcel.2007.11.021
Gaur, Rahul ; Grasso, Domenick ; Datta, Partha P. ; Krishna, P. D.V. ; Das, Gautam ; Spencer, Angela C ; Agrawal, Rajendra K. ; Spremulli, Linda ; Varshney, Umesh. / A Single Mammalian Mitochondrial Translation Initiation Factor Functionally Replaces Two Bacterial Factors. In: Molecular Cell. 2008 ; Vol. 29, No. 2. pp. 180-190.
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