A somatic cell hybrid map of human chromosome 13

Sarah S. Washington, Anne M. Bowcock, Steve Gerken, Norisada Matsunami, David Lesh, Sherri L. Osborne-Lawrence, John Kenneth Cowell, David H. Ledbetter, Raymond L. White, Aravinda Chakravarti

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We have constructed a chromosome 13 somatic cell hybrid map using seven cell lines: PGMEA6, a hybrid containing the entire chromosome 13, and six hybrids containing various deletions of chromosomes 13 (BARF7, PPF22, KBF11, KSF39, CF25, and CF27). We have mapped 80 markers that define 10 regions of chromosome 13 with respect to 10 breakpoints in the mapping panel; these regions range in size from 4 to 24 Mb, with an average size of 8 Mb. The 80 markers sublocalized on our mapping panel include 10 Alu-PCR clones, 6 of which were converted to sequence-tagged sites; 40 (CA)(n) repeat-containing clones, 27 of which are micro-satellite PCR markers; 8 (AAAG)(n) repeat- containing PCR markers, 1 two-allele PCR marker, 4 genes or expressed sequences, and 17 anonymous DNA probes. This low-resolution physical map can be used as a backbone map for more refined physical mapping using radiation hybrids or yeast artificial chromosomes.

Original languageEnglish (US)
Pages (from-to)486-495
Number of pages10
JournalGenomics
Volume18
Issue number3
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Washington, S. S., Bowcock, A. M., Gerken, S., Matsunami, N., Lesh, D., Osborne-Lawrence, S. L., Cowell, J. K., Ledbetter, D. H., White, R. L., & Chakravarti, A. (1993). A somatic cell hybrid map of human chromosome 13. Genomics, 18(3), 486-495. https://doi.org/10.1016/S0888-7543(11)80004-2