A stress-dependent SUMO4 sumoylation of its substrate proteins

Wenzhong Wei, Ping Yang, Junfeng Pang, Shu Zhang, Ying Wang, Mong-Heng Wang, Zheng Dong, Jin-Xiong She, Cong Yi Wang

Research output: Contribution to journalArticle

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Abstract

Here we performed studies to demonstrate SUMO4 maturation process. Unlike other SUMO proteins, cells under physiological condition mediate a rapid degradation for SUMO4. However, when cells under stressed condition, SUMO4 can be matured by the stress-induced endogenous hydrolase and be able to covalently conjugate to its substrate proteins. Furthermore, we failed to obtain evidence supporting a role for proline-90 unique to SUMO4 in its activation and functionality. Both wild-type SUMO4 and SUMO4-P90Q can be hydrolyzed by the stressed RAW264.7 cell lysates, and no significant functional difference between SUMO4, SUMO4-P90Q, and SUMO4-GG (matured form) was observed as determined by luciferase assay. However, the C-terminal di-glycine motif, a prerequisite for sumoylation, is necessary for SUMO4 to exert its functional activity. These data not only confirmed our previous published data, but also provided additional evidence suggesting a role for SUMO4 sumoylation in the regulation of intracellular stress.

Original languageEnglish (US)
Pages (from-to)454-459
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume375
Issue number3
DOIs
StatePublished - Oct 24 2008

Fingerprint

Sumoylation
Small Ubiquitin-Related Modifier Proteins
Hydrolases
Substrates
Luciferases
Proline
Glycine
Assays
Proteins
Chemical activation
Degradation

Keywords

  • Hydrolase
  • Intracellular stress
  • Maturation
  • SUMO4
  • Serum starvation
  • Sumoylation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

A stress-dependent SUMO4 sumoylation of its substrate proteins. / Wei, Wenzhong; Yang, Ping; Pang, Junfeng; Zhang, Shu; Wang, Ying; Wang, Mong-Heng; Dong, Zheng; She, Jin-Xiong; Wang, Cong Yi.

In: Biochemical and Biophysical Research Communications, Vol. 375, No. 3, 24.10.2008, p. 454-459.

Research output: Contribution to journalArticle

Wei, Wenzhong ; Yang, Ping ; Pang, Junfeng ; Zhang, Shu ; Wang, Ying ; Wang, Mong-Heng ; Dong, Zheng ; She, Jin-Xiong ; Wang, Cong Yi. / A stress-dependent SUMO4 sumoylation of its substrate proteins. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 375, No. 3. pp. 454-459.
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