A study on neuroinflammatory marker in brain areas of okadaic acid (ICV) induced memory impaired rats

Pradeep Kumar Kamat, Santoshkumar Tota, Shivika Rai, Supriya Swarnkar, Rakesh Shukla, Chandishwar Nath

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Aims: The aim of the present study is to investigate the status of proinflammatory cytokine in the brain of intracerebroventricular (ICV) okadaic acid (OKA) induced memory impaired rat. Main methods: OKA (200 ng) intracerebroventricular (ICV) was administered in rats. Memory was assessed by Morris water maze test. Biochemical marker of neuroinflammation (TNF-α, IL-β), total nitrite, mRNA (RT PCR) and protein expression (WB) of iNOS and nNOS were estimated in rat brain areas. Key findings: OKA caused memory-impairment in rats with increased expression of proinflammatory cytokine TNF-α and IL-1β and total nitrite in brain regions hippocampus and cortex. The expression of mRNA and protein of iNOS was increased while; the expressions were decreased in case of nNOS. Pretreatment with antidementic drugs donepezil (5 mg/kg, p.o.) and memantine (10 mg/kg, p.o) for 13 days protected ICV OKA induced memory impairment and changes in level of TNF-α, IL-β, total nitrite and expressions of iNOS and nNOS in OKA treated rat. Significance: This study suggests that neuroinflammation may play a vital role in OKA induced memory impairment.

Original languageEnglish (US)
Pages (from-to)713-720
Number of pages8
JournalLife sciences
Volume90
Issue number19-20
DOIs
StatePublished - May 22 2012
Externally publishedYes

Keywords

  • Donepezil
  • Memantine
  • Memory
  • Neuroinflammation
  • Nitric oxide synthase
  • Okadaic acid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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