A suite of novel human spermatozoal RNAs

G. Charles Ostermeier, Robert J. Goodrich, Julie S. Moldenhauer, Michael P. Diamond, Stephen A. Krawetz

Research output: Contribution to journalArticle

153 Scopus citations

Abstract

We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.

Original languageEnglish (US)
Pages (from-to)70-74
Number of pages5
JournalJournal of Andrology
Volume26
Issue number1
StatePublished - Jan 1 2005
Externally publishedYes

Keywords

  • Antisense RNA
  • Cloning
  • Development
  • Fertility
  • Microarray
  • Novel transcripts
  • Spermatozoa

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Urology

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  • Cite this

    Ostermeier, G. C., Goodrich, R. J., Moldenhauer, J. S., Diamond, M. P., & Krawetz, S. A. (2005). A suite of novel human spermatozoal RNAs. Journal of Andrology, 26(1), 70-74.