A suite of novel human spermatozoal RNAs

G. Charles Ostermeier, Robert J. Goodrich, Julie S. Moldenhauer, Michael Peter Diamond, Stephen A. Krawetz

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.

Original languageEnglish (US)
Pages (from-to)70-74
Number of pages5
JournalJournal of Andrology
Volume26
Issue number1
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

RNA
MicroRNAs
Spermatozoa
Antisense RNA
Spermatogenesis
Oligonucleotide Array Sequence Analysis
Fertilization
Computer Simulation
Oocytes
Testis
Population

Keywords

  • Antisense RNA
  • Cloning
  • Development
  • Fertility
  • Microarray
  • Novel transcripts
  • Spermatozoa

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Urology

Cite this

Ostermeier, G. C., Goodrich, R. J., Moldenhauer, J. S., Diamond, M. P., & Krawetz, S. A. (2005). A suite of novel human spermatozoal RNAs. Journal of Andrology, 26(1), 70-74.

A suite of novel human spermatozoal RNAs. / Ostermeier, G. Charles; Goodrich, Robert J.; Moldenhauer, Julie S.; Diamond, Michael Peter; Krawetz, Stephen A.

In: Journal of Andrology, Vol. 26, No. 1, 01.01.2005, p. 70-74.

Research output: Contribution to journalArticle

Ostermeier, GC, Goodrich, RJ, Moldenhauer, JS, Diamond, MP & Krawetz, SA 2005, 'A suite of novel human spermatozoal RNAs', Journal of Andrology, vol. 26, no. 1, pp. 70-74.
Ostermeier GC, Goodrich RJ, Moldenhauer JS, Diamond MP, Krawetz SA. A suite of novel human spermatozoal RNAs. Journal of Andrology. 2005 Jan 1;26(1):70-74.
Ostermeier, G. Charles ; Goodrich, Robert J. ; Moldenhauer, Julie S. ; Diamond, Michael Peter ; Krawetz, Stephen A. / A suite of novel human spermatozoal RNAs. In: Journal of Andrology. 2005 ; Vol. 26, No. 1. pp. 70-74.
@article{09716ccd0ea44eda92f3c492347b7279,
title = "A suite of novel human spermatozoal RNAs",
abstract = "We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.",
keywords = "Antisense RNA, Cloning, Development, Fertility, Microarray, Novel transcripts, Spermatozoa",
author = "Ostermeier, {G. Charles} and Goodrich, {Robert J.} and Moldenhauer, {Julie S.} and Diamond, {Michael Peter} and Krawetz, {Stephen A.}",
year = "2005",
month = "1",
day = "1",
language = "English (US)",
volume = "26",
pages = "70--74",
journal = "Journal of Andrology",
issn = "0196-3635",
publisher = "American Society of Andrology Inc.",
number = "1",

}

TY - JOUR

T1 - A suite of novel human spermatozoal RNAs

AU - Ostermeier, G. Charles

AU - Goodrich, Robert J.

AU - Moldenhauer, Julie S.

AU - Diamond, Michael Peter

AU - Krawetz, Stephen A.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.

AB - We recently described a complex population of spermatozoal coding RNAs that are delivered to the oocyte on fertilization. These are derived throughout spermatogenesis, representing a record of past events. Recently, evidence has been provided that micro-RNAs are present in testes, suggesting that they might also be carried in ejaculate spermatozoa. To directly test this hypothesis, a unique microarray system capable of directly identifying antisense RNAs and predicted transcripts was utilized. RNA isolated from the ejaculate spermatozoa of 6 normal fertile men was directly hybridized to sense oligonucleotide arrays containing 10000 elements. This revealed 68 shared RNAs, some of which are similar to those previously defined as micro-RNAs, whereas others were the antisense of previously in silico-predicted transcripts. The results and implications of this study are described in this communication.

KW - Antisense RNA

KW - Cloning

KW - Development

KW - Fertility

KW - Microarray

KW - Novel transcripts

KW - Spermatozoa

UR - http://www.scopus.com/inward/record.url?scp=10844236571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10844236571&partnerID=8YFLogxK

M3 - Article

VL - 26

SP - 70

EP - 74

JO - Journal of Andrology

JF - Journal of Andrology

SN - 0196-3635

IS - 1

ER -