A survey of small RNAs in human sperm

Stephen A. Krawetz, Adele Kruger, Claudia Lalancette, Rebecca Tagett, Ester Anton, Sorin Draghici, Michael Peter Diamond

Research output: Contribution to journalArticle

200 Citations (Scopus)

Abstract

Background: There has been substantial interest in assessing whether RNAs (mRNAs and sncRNAs, i.e. small non-coding) delivered from mammalian spermatozoa play a functional role in early embryo development. While the cadre of spermatozoal mRNAs has been characterized, comparatively little is known about the distribution or function of the estimated 24 000 sncRNAs within each normal human spermatozoon.Methods: RNAs of <200 bases in length were isolated from the ejaculates from three donors of proved fertility. RNAs of 1830 nucleotides in length were then used to construct small RNA Digital Gene Expression libraries for Next Generation Sequencing. Known sncRNAs that uniquely mapped to a single location in the human genome were identified.Results: Bioinformatic analysis revealed the presence of multiple classes of small RNAs in human spermatozoa. The primary classes resolved included microRNA (miRNAs) (≈7), Piwi-interacting piRNAs (≈17), repeat-associated small RNAs (≈65). A minor subset of short RNAs within the transcription start site/promoter fraction (≈11) frames the histone promoter-associated regions enriched in genes of early embryonic development. These have been termed quiescent RNAs. Conclusions: A complex population of male derived sncRNAs that are available for delivery upon fertilization was revealed. Sperm miRNA-targeted enrichment in the human oocyte is consistent with their role as modifiers of early post-fertilization. The relative abundance of piRNAs and repeat-associated RNAs suggests that they may assume a role in confrontation and consolidation. This may ensure the compatibility of the genomes at fertilization.

Original languageEnglish (US)
Pages (from-to)3401-3412
Number of pages12
JournalHuman Reproduction
Volume26
Issue number12
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Fingerprint

Spermatozoa
RNA
Small Untranslated RNA
Fertilization
MicroRNAs
Embryonic Development
Surveys and Questionnaires
Messenger RNA
Transcription Initiation Site
Human Genome
Computational Biology
Gene Library
Genetic Promoter Regions
Histones
Oocytes
Fertility
Nucleotides
Genome
Gene Expression
Population

Keywords

  • microRNA
  • Piwi-interacting RNAs RNA
  • small non-coding RNA
  • spermatozoa

ASJC Scopus subject areas

  • Reproductive Medicine
  • Rehabilitation
  • Obstetrics and Gynecology

Cite this

Krawetz, S. A., Kruger, A., Lalancette, C., Tagett, R., Anton, E., Draghici, S., & Diamond, M. P. (2011). A survey of small RNAs in human sperm. Human Reproduction, 26(12), 3401-3412. https://doi.org/10.1093/humrep/der329

A survey of small RNAs in human sperm. / Krawetz, Stephen A.; Kruger, Adele; Lalancette, Claudia; Tagett, Rebecca; Anton, Ester; Draghici, Sorin; Diamond, Michael Peter.

In: Human Reproduction, Vol. 26, No. 12, 01.12.2011, p. 3401-3412.

Research output: Contribution to journalArticle

Krawetz, SA, Kruger, A, Lalancette, C, Tagett, R, Anton, E, Draghici, S & Diamond, MP 2011, 'A survey of small RNAs in human sperm', Human Reproduction, vol. 26, no. 12, pp. 3401-3412. https://doi.org/10.1093/humrep/der329
Krawetz SA, Kruger A, Lalancette C, Tagett R, Anton E, Draghici S et al. A survey of small RNAs in human sperm. Human Reproduction. 2011 Dec 1;26(12):3401-3412. https://doi.org/10.1093/humrep/der329
Krawetz, Stephen A. ; Kruger, Adele ; Lalancette, Claudia ; Tagett, Rebecca ; Anton, Ester ; Draghici, Sorin ; Diamond, Michael Peter. / A survey of small RNAs in human sperm. In: Human Reproduction. 2011 ; Vol. 26, No. 12. pp. 3401-3412.
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