A transformed murine Leydig cell line expresses the ETA receptor subtype

Adviye Ergul, Marilyn K. Glassberg, Marc E. Freeman, David Puett

Research output: Contribution to journalArticle

Abstract

We recently demonstrated that transformed murine Leydig cells (MA-10) responded to endothelin-1 (ET-1) via increased steroidogenesis. This study addresses the endothelin receptor subtype present on this cell line and whether or not the cells produce ET-1. The expression of the preproendothelin-1 (PPET-1) gene was investigated by Northern blot analysis, and PPET-1 mRNA was found to be <0.2% of that present in pulmonary endothelial cells. The medium from MA-10 cells, maintained under serum-free conditions, was analyzed by radio-immunoassay to determine immunoreactive-ET-1 production and ET-1 levels were found to be below the sensitivity of the assay (<10 pg/ml). The data from competitive binding experiments with [125I]ET-1 and unlabeled ET-1, ET-3 and receptor subtype selective ligands yielded a single class of high affinity binding sites with ETA receptor subtype characteristics. The results of this study demonstrate that MA-10 cells possess the ETA receptor subtype but do not produce significant quantities of ET-1 under basal conditions.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume136
Issue number1
DOIs
StatePublished - Jul 1 1994

Keywords

  • Leydig tumor cells
  • endothelin receptor
  • endothelin-1

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'A transformed murine Leydig cell line expresses the ET<sub>A</sub> receptor subtype'. Together they form a unique fingerprint.

  • Cite this