A transformed murine Leydig cell line expresses the ETA receptor subtype

Adviye Ergul, Marilyn K. Glassberg, Marc E. Freeman, David Puett

Research output: Contribution to journalArticle

Abstract

We recently demonstrated that transformed murine Leydig cells (MA-10) responded to endothelin-1 (ET-1) via increased steroidogenesis. This study addresses the endothelin receptor subtype present on this cell line and whether or not the cells produce ET-1. The expression of the preproendothelin-1 (PPET-1) gene was investigated by Northern blot analysis, and PPET-1 mRNA was found to be <0.2% of that present in pulmonary endothelial cells. The medium from MA-10 cells, maintained under serum-free conditions, was analyzed by radio-immunoassay to determine immunoreactive-ET-1 production and ET-1 levels were found to be below the sensitivity of the assay (<10 pg/ml). The data from competitive binding experiments with [125I]ET-1 and unlabeled ET-1, ET-3 and receptor subtype selective ligands yielded a single class of high affinity binding sites with ETA receptor subtype characteristics. The results of this study demonstrate that MA-10 cells possess the ETA receptor subtype but do not produce significant quantities of ET-1 under basal conditions.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume136
Issue number1
DOIs
StatePublished - Jul 1 1994
Externally publishedYes

Fingerprint

Leydig Cells
Endothelin-1
Cells
Cell Line
Endothelin A Receptors
Endothelin Receptors
Competitive Binding
Radio
Endothelial cells
Immunoassay
Northern Blotting
Endothelial Cells
Assays
Binding Sites
Genes
Ligands
Lung
Messenger RNA
Serum

Keywords

  • endothelin receptor
  • endothelin-1
  • Leydig tumor cells

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

A transformed murine Leydig cell line expresses the ETA receptor subtype. / Ergul, Adviye; Glassberg, Marilyn K.; Freeman, Marc E.; Puett, David.

In: Molecular and Cellular Biochemistry, Vol. 136, No. 1, 01.07.1994, p. 91-96.

Research output: Contribution to journalArticle

Ergul, Adviye ; Glassberg, Marilyn K. ; Freeman, Marc E. ; Puett, David. / A transformed murine Leydig cell line expresses the ETA receptor subtype. In: Molecular and Cellular Biochemistry. 1994 ; Vol. 136, No. 1. pp. 91-96.
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