A transient rice in cytosolic calcium follows stimulation of quiescent cells with growth factors and is inhibitable with phorbol myristate acetate

Paul L McNeil, Michael P. McKenna, D. Lansing Taylor

Research output: Contribution to journalArticle

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Abstract

We have used aequorin as an indicator for the intracellular free calcium ion concentration ([Ca++]i) of Swiss 3T3 fibroblasts. Estimated [Ca++]i of serum-deprived, subconfluent fibroblasts was 89 (±20) nM, almost twofold higher than that of subconfluent cells growing in serum, whose [Ca++]i was 50 (±19) nM. Serum, partially purified platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) stimulated DNA synthesis by the serum-deprived cells, whereas epidermal growth factor (EGF) did not. Serum immediately and transiently elevated the [Ca++]i of serum-deprived cells, which reached a maximal value of 5.3 µM at 18 s poststimulation but returned to near prestimulatory levels within 3 min. Moreover, no further changes in [Ca++]i were observed during 12 subsequent h of continuous recording. PDGF produced a peak rise in [Ca++]i to ∼1.4 pM at 115 s after stimulation, and FGF to ∼1.2 µM at 135 s after stimulation. EGF caused no change in [Ca++]i. The primary source of calcium for these transients was intracellular, since the magnitude of the seruminduced rise in [Ca++]i was reduced by only 30% in the absence of exogenous calcium. Phorbol 12-myristate 13-acetate (PMA) had no effect on resting [Ca++]i. When, however, quiescent cells were treated for 30 min with 100 nM PMA, serum-induced rises in [Ca++]i were reduced by sevenfold. PMA did not inhibit growth factor-induced DNA synthesis and was by itself partially mitogenic. We suggest that if calcium is involved as a cytoplasmic signal for mitogenic activation of quiescent fibroblasts, its action is early, transient, and can be partially substituted for by PMA. Activated protein kinase C may regulate growth factor-induced increases in [Ca++]i.

Original languageEnglish (US)
Pages (from-to)372-379
Number of pages8
JournalJournal of Cell Biology
Volume101
Issue number2
DOIs
StatePublished - Aug 1 1985

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Tetradecanoylphorbol Acetate
Intercellular Signaling Peptides and Proteins
Calcium
Serum
Acetates
Fibroblast Growth Factors
Fibroblasts
Platelet-Derived Growth Factor
Epidermal Growth Factor
Aequorin
DNA
Oryza
Protein Kinase C
Ions
phorbol-12-myristate

ASJC Scopus subject areas

  • Cell Biology

Cite this

A transient rice in cytosolic calcium follows stimulation of quiescent cells with growth factors and is inhibitable with phorbol myristate acetate. / McNeil, Paul L; McKenna, Michael P.; Lansing Taylor, D.

In: Journal of Cell Biology, Vol. 101, No. 2, 01.08.1985, p. 372-379.

Research output: Contribution to journalArticle

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abstract = "We have used aequorin as an indicator for the intracellular free calcium ion concentration ([Ca++]i) of Swiss 3T3 fibroblasts. Estimated [Ca++]i of serum-deprived, subconfluent fibroblasts was 89 (±20) nM, almost twofold higher than that of subconfluent cells growing in serum, whose [Ca++]i was 50 (±19) nM. Serum, partially purified platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) stimulated DNA synthesis by the serum-deprived cells, whereas epidermal growth factor (EGF) did not. Serum immediately and transiently elevated the [Ca++]i of serum-deprived cells, which reached a maximal value of 5.3 µM at 18 s poststimulation but returned to near prestimulatory levels within 3 min. Moreover, no further changes in [Ca++]i were observed during 12 subsequent h of continuous recording. PDGF produced a peak rise in [Ca++]i to ∼1.4 pM at 115 s after stimulation, and FGF to ∼1.2 µM at 135 s after stimulation. EGF caused no change in [Ca++]i. The primary source of calcium for these transients was intracellular, since the magnitude of the seruminduced rise in [Ca++]i was reduced by only 30{\%} in the absence of exogenous calcium. Phorbol 12-myristate 13-acetate (PMA) had no effect on resting [Ca++]i. When, however, quiescent cells were treated for 30 min with 100 nM PMA, serum-induced rises in [Ca++]i were reduced by sevenfold. PMA did not inhibit growth factor-induced DNA synthesis and was by itself partially mitogenic. We suggest that if calcium is involved as a cytoplasmic signal for mitogenic activation of quiescent fibroblasts, its action is early, transient, and can be partially substituted for by PMA. Activated protein kinase C may regulate growth factor-induced increases in [Ca++]i.",
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