A transient rice in cytosolic calcium follows stimulation of quiescent cells with growth factors and is inhibitable with phorbol myristate acetate

Paul L. McNeil, Michael P. McKenna, D. Lansing Taylor

Research output: Contribution to journalArticle

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Abstract

We have used aequorin as an indicator for the intracellular free calcium ion concentration ([Ca++]i) of Swiss 3T3 fibroblasts. Estimated [Ca++]i of serum-deprived, subconfluent fibroblasts was 89 (±20) nM, almost twofold higher than that of subconfluent cells growing in serum, whose [Ca++]i was 50 (±19) nM. Serum, partially purified platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) stimulated DNA synthesis by the serum-deprived cells, whereas epidermal growth factor (EGF) did not. Serum immediately and transiently elevated the [Ca++]i of serum-deprived cells, which reached a maximal value of 5.3 µM at 18 s poststimulation but returned to near prestimulatory levels within 3 min. Moreover, no further changes in [Ca++]i were observed during 12 subsequent h of continuous recording. PDGF produced a peak rise in [Ca++]i to ∼1.4 pM at 115 s after stimulation, and FGF to ∼1.2 µM at 135 s after stimulation. EGF caused no change in [Ca++]i. The primary source of calcium for these transients was intracellular, since the magnitude of the seruminduced rise in [Ca++]i was reduced by only 30% in the absence of exogenous calcium. Phorbol 12-myristate 13-acetate (PMA) had no effect on resting [Ca++]i. When, however, quiescent cells were treated for 30 min with 100 nM PMA, serum-induced rises in [Ca++]i were reduced by sevenfold. PMA did not inhibit growth factor-induced DNA synthesis and was by itself partially mitogenic. We suggest that if calcium is involved as a cytoplasmic signal for mitogenic activation of quiescent fibroblasts, its action is early, transient, and can be partially substituted for by PMA. Activated protein kinase C may regulate growth factor-induced increases in [Ca++]i.

Original languageEnglish (US)
Pages (from-to)372-379
Number of pages8
JournalJournal of Cell Biology
Volume101
Issue number2
DOIs
StatePublished - Aug 1 1985

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Tetradecanoylphorbol Acetate
Intercellular Signaling Peptides and Proteins
Calcium
Serum
Acetates
Fibroblast Growth Factors
Fibroblasts
Platelet-Derived Growth Factor
Epidermal Growth Factor
Aequorin
DNA
Oryza
Protein Kinase C
Ions
phorbol-12-myristate

ASJC Scopus subject areas

  • Cell Biology

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A transient rice in cytosolic calcium follows stimulation of quiescent cells with growth factors and is inhibitable with phorbol myristate acetate. / McNeil, Paul L.; McKenna, Michael P.; Lansing Taylor, D.

In: Journal of Cell Biology, Vol. 101, No. 2, 01.08.1985, p. 372-379.

Research output: Contribution to journalArticle

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abstract = "We have used aequorin as an indicator for the intracellular free calcium ion concentration ([Ca++]i) of Swiss 3T3 fibroblasts. Estimated [Ca++]i of serum-deprived, subconfluent fibroblasts was 89 (±20) nM, almost twofold higher than that of subconfluent cells growing in serum, whose [Ca++]i was 50 (±19) nM. Serum, partially purified platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF) stimulated DNA synthesis by the serum-deprived cells, whereas epidermal growth factor (EGF) did not. Serum immediately and transiently elevated the [Ca++]i of serum-deprived cells, which reached a maximal value of 5.3 µM at 18 s poststimulation but returned to near prestimulatory levels within 3 min. Moreover, no further changes in [Ca++]i were observed during 12 subsequent h of continuous recording. PDGF produced a peak rise in [Ca++]i to ∼1.4 pM at 115 s after stimulation, and FGF to ∼1.2 µM at 135 s after stimulation. EGF caused no change in [Ca++]i. The primary source of calcium for these transients was intracellular, since the magnitude of the seruminduced rise in [Ca++]i was reduced by only 30{\%} in the absence of exogenous calcium. Phorbol 12-myristate 13-acetate (PMA) had no effect on resting [Ca++]i. When, however, quiescent cells were treated for 30 min with 100 nM PMA, serum-induced rises in [Ca++]i were reduced by sevenfold. PMA did not inhibit growth factor-induced DNA synthesis and was by itself partially mitogenic. We suggest that if calcium is involved as a cytoplasmic signal for mitogenic activation of quiescent fibroblasts, its action is early, transient, and can be partially substituted for by PMA. Activated protein kinase C may regulate growth factor-induced increases in [Ca++]i.",
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