A Triple Arg Motif Mediates α 2B-Adrenergic Receptor Interaction with Sec24C/D and Export

Chunmin Dong, Charles D. Nichols, Jianhui Guo, Wei Huang, Nevin A Lambert, Guangyu Wu

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Recent studies have demonstrated that cargo exit from the endoplasmic reticulum (ER) may be directed by ER export motifs recognized by components of the coat protein II (COPII) vesicles. However, little is known about ER export motifs and vesicle targeting of the G protein-coupled receptor (GPCR) superfamily. Here, we have demonstrated that a triple Arg (3R) motif in the third intracellular loop functions as a novel ER export signal for α 2B-adrenergic receptor (α 2B-AR). The 3R motif mediates α 2B-AR interaction with Sec24C/D and modulates ER exit, cell surface transport and function of α 2B-AR. Furthermore, export function of the 3R motif is independent of its position within α 2B-AR and can be conferred to CD8 glycoprotein. These data provide the first evidence implicating that export of GPCRs is controlled by code-directed interactions with selective components of the COPII transport machinery.

Original languageEnglish (US)
Pages (from-to)857-868
Number of pages12
JournalTraffic
Volume13
Issue number6
DOIs
StatePublished - Jun 1 2012

Keywords

  • Adrenergic receptor
  • COPII vesicle
  • Cell surface transport
  • Endoplasmic reticulum
  • Export motif
  • G protein-coupled receptor
  • Intracellular loop
  • Sec24

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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