A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells: A Comparative Lipidomic Study

Yutaka Itokazu, Nobuyoshi Tajima, Laura Kerosuo, Pentti Somerharju, Hannu Sariola, Robert K Yu, Reijo Käkelä

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The central nervous system (CNS) harbors multiple glial fibrillary acidic protein (GFAP) expressing cell types. In addition to the most abundant cell type of the CNS, the astrocytes, various stem cells and progenitor cells also contain GFAP+ populations. Here, in order to distinguish between two types of GFAP expressing cells with or without the expression of the A2B5 antigens, we performed lipidomic analyses on A2B5+/GFAP+ and A2B5−/GFAP+ cells from rat spinal cord. First, A2B5+/GFAP− progenitors were exposed to the leukemia inhibitory factor (LIF) or bone morphogenetic protein (BMP) to induce their differentiation to A2B5+/GFAP+ cells or A2B5−/GFAP+ astrocytes, respectively. The cells were then analyzed for changes in their phospholipid, sphingolipid or acyl chain profiles by mass spectrometry and gas chromatography. Compared to A2B5+/GFAP− progenitors, A2B5−/GFAP+ astrocytes contained higher amounts of ether phospholipids (especially the species containing arachidonic acid) and sphingomyelin, which may indicate characteristics of cellular differentiation and inability for multipotency. In comparison, principal component analyses revealed that the lipid composition of A2B5+/GFAP+ cells retained many of the characteristics of A2B5+/GFAP− progenitors, but their lipid profile was different from that of A2B5−/GFAP+ astrocytes. Thus, our study demonstrated that two GFAP+ cell populations have distinct lipid profiles with the A2B5+/GFAP+ cells sharing a phospholipid profile with progenitors rather than astrocytes. The progenitor cells may require regulated low levels of lipids known to mediate signaling functions in differentiated cells, and the precursor lipid profiles may serve as one measure of the differentiation capacity of a cell population.

Original languageEnglish (US)
Pages (from-to)1527-1544
Number of pages18
JournalNeurochemical Research
Volume41
Issue number7
DOIs
StatePublished - Jul 1 2016

Fingerprint

Glial Fibrillary Acidic Protein
Rats
Spinal Cord
Stem Cells
Cells
Lipids
Astrocytes
Neurology
Phospholipids
Phospholipid Ethers
Central Nervous System
Population
Leukemia Inhibitory Factor
Bone Morphogenetic Proteins
Sphingolipids
Sphingomyelins
Ports and harbors
Principal Component Analysis
Stem cells
Arachidonic Acid

Keywords

  • A2B5 antigen
  • Astrocyte
  • Glial fibrillary acidic protein
  • Glial progenitor/precursor cell
  • Lipidomics
  • Mass spectrometry
  • Phospholipid
  • Sphingolipid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study. / Itokazu, Yutaka; Tajima, Nobuyoshi; Kerosuo, Laura; Somerharju, Pentti; Sariola, Hannu; Yu, Robert K; Käkelä, Reijo.

In: Neurochemical Research, Vol. 41, No. 7, 01.07.2016, p. 1527-1544.

Research output: Contribution to journalArticle

Itokazu, Yutaka ; Tajima, Nobuyoshi ; Kerosuo, Laura ; Somerharju, Pentti ; Sariola, Hannu ; Yu, Robert K ; Käkelä, Reijo. / A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells : A Comparative Lipidomic Study. In: Neurochemical Research. 2016 ; Vol. 41, No. 7. pp. 1527-1544.
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