Ability of bacterial endotoxin to diffuse through human dentin

Roni Nissan, Hilton Segal, David Henry Pashley, Roy Stevens, Henry Trowbridge

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

An in vitro system was developed to determine whether bacterial endotoxin is capable of diffusing through dentin without the use of filtration pressure. Cavities were prepared in five third molar teeth in order to produce a split chamber device consisting of occlusal and pulpal chambers with 0.5 mm of intervening dentin. Endotoxin was introduced into the occlusal chamber and the effluent in the pulpal chamber was sampled every 30 min for 5 h and at 24 h using the limulus lysate assay. In four specimens the initial appearance of endotoxin in the effluent ranged from 15 min to 41/2 h. In two specimens the concentration of endotoxin in the effluent leveled off in 41/2 and 5 h, respectively, whereas in another two the concentration continued to increase throughout the experiment. In one specimen no endotoxin was detected. The results indicate that endotoxin is capable of passing through 0.5 mm of dentin.

Original languageEnglish (US)
Pages (from-to)62-64
Number of pages3
JournalJournal of Endodontics
Volume21
Issue number2
DOIs
StatePublished - Jan 1 1995

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Dentin
Endotoxins
Horseshoe Crabs
Third Molar
Tooth
Pressure
Equipment and Supplies

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Nissan, R., Segal, H., Pashley, D. H., Stevens, R., & Trowbridge, H. (1995). Ability of bacterial endotoxin to diffuse through human dentin. Journal of Endodontics, 21(2), 62-64. https://doi.org/10.1016/S0099-2399(06)81096-4

Ability of bacterial endotoxin to diffuse through human dentin. / Nissan, Roni; Segal, Hilton; Pashley, David Henry; Stevens, Roy; Trowbridge, Henry.

In: Journal of Endodontics, Vol. 21, No. 2, 01.01.1995, p. 62-64.

Research output: Contribution to journalArticle

Nissan, R, Segal, H, Pashley, DH, Stevens, R & Trowbridge, H 1995, 'Ability of bacterial endotoxin to diffuse through human dentin', Journal of Endodontics, vol. 21, no. 2, pp. 62-64. https://doi.org/10.1016/S0099-2399(06)81096-4
Nissan, Roni ; Segal, Hilton ; Pashley, David Henry ; Stevens, Roy ; Trowbridge, Henry. / Ability of bacterial endotoxin to diffuse through human dentin. In: Journal of Endodontics. 1995 ; Vol. 21, No. 2. pp. 62-64.
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