Abstract
An in vitro system was developed to determine whether bacterial endotoxin is capable of diffusing through dentin without the use of filtration pressure. Cavities were prepared in five third molar teeth in order to produce a split chamber device consisting of occlusal and pulpal chambers with 0.5 mm of intervening dentin. Endotoxin was introduced into the occlusal chamber and the effluent in the pulpal chamber was sampled every 30 min for 5 h and at 24 h using the limulus lysate assay. In four specimens the initial appearance of endotoxin in the effluent ranged from 15 min to 41/2 h. In two specimens the concentration of endotoxin in the effluent leveled off in 41/2 and 5 h, respectively, whereas in another two the concentration continued to increase throughout the experiment. In one specimen no endotoxin was detected. The results indicate that endotoxin is capable of passing through 0.5 mm of dentin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 62-64 |
| Number of pages | 3 |
| Journal | Journal of Endodontics |
| Volume | 21 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1995 |
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ASJC Scopus subject areas
- Dentistry(all)
Cite this
Ability of bacterial endotoxin to diffuse through human dentin. / Nissan, Roni; Segal, Hilton; Pashley, David Henry; Stevens, Roy; Trowbridge, Henry.
In: Journal of Endodontics, Vol. 21, No. 2, 01.01.1995, p. 62-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ability of bacterial endotoxin to diffuse through human dentin
AU - Nissan, Roni
AU - Segal, Hilton
AU - Pashley, David Henry
AU - Stevens, Roy
AU - Trowbridge, Henry
PY - 1995/1/1
Y1 - 1995/1/1
N2 - An in vitro system was developed to determine whether bacterial endotoxin is capable of diffusing through dentin without the use of filtration pressure. Cavities were prepared in five third molar teeth in order to produce a split chamber device consisting of occlusal and pulpal chambers with 0.5 mm of intervening dentin. Endotoxin was introduced into the occlusal chamber and the effluent in the pulpal chamber was sampled every 30 min for 5 h and at 24 h using the limulus lysate assay. In four specimens the initial appearance of endotoxin in the effluent ranged from 15 min to 41/2 h. In two specimens the concentration of endotoxin in the effluent leveled off in 41/2 and 5 h, respectively, whereas in another two the concentration continued to increase throughout the experiment. In one specimen no endotoxin was detected. The results indicate that endotoxin is capable of passing through 0.5 mm of dentin.
AB - An in vitro system was developed to determine whether bacterial endotoxin is capable of diffusing through dentin without the use of filtration pressure. Cavities were prepared in five third molar teeth in order to produce a split chamber device consisting of occlusal and pulpal chambers with 0.5 mm of intervening dentin. Endotoxin was introduced into the occlusal chamber and the effluent in the pulpal chamber was sampled every 30 min for 5 h and at 24 h using the limulus lysate assay. In four specimens the initial appearance of endotoxin in the effluent ranged from 15 min to 41/2 h. In two specimens the concentration of endotoxin in the effluent leveled off in 41/2 and 5 h, respectively, whereas in another two the concentration continued to increase throughout the experiment. In one specimen no endotoxin was detected. The results indicate that endotoxin is capable of passing through 0.5 mm of dentin.
UR - http://www.scopus.com/inward/record.url?scp=0029246584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029246584&partnerID=8YFLogxK
U2 - 10.1016/S0099-2399(06)81096-4
DO - 10.1016/S0099-2399(06)81096-4
M3 - Article
VL - 21
SP - 62
EP - 64
JO - Journal of Endodontics
JF - Journal of Endodontics
SN - 0099-2399
IS - 2
ER -