Abrogation of estrogen receptor signaling augments cytotoxicity of anticancer drugs on caski cervical cancer cells

Moon Y. Heo, Salama A. Salama, Nilufar Khatoon, Ayman Al-Hendy, William W. Au

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: We have reported that a gene therapy approach, using a dominant-negative estrogen receptor blocker (DN) gene, can cause cell death in cervical cancer cells in vitro. We investigated the mechanisms for enhanced cell killing when DN was combined with cisplatin (CP) and paclitaxel (TX). Materials and Methods: Cells were transduced with DN at 24 h and/or treated with drugs at 48 h, and harvested at 48 and 72 h after transduction. Effects were determined using the MTT cytotoxic, and TUNEL and caspase-3 activity apoptotic assays. Results: Each agent induced cytotoxic and apoptotic effects, and activated caspase-3. In the combined treatments, significant synergistic effects were observed based on the MTT and TUNEL assays, but with antagonistic caspase-3 activation effect. Conclusion: The enhanced cell killing effect was mediated by the initiation of new and multiple mechanisms, particularly via caspase-independent pathways.

Original languageEnglish (US)
Pages (from-to)2181-2187
Number of pages7
JournalAnticancer research
Volume28
Issue number4 B
StatePublished - Jul 1 2008

Fingerprint

Uterine Cervical Neoplasms
Estrogen Receptors
Caspase 3
In Situ Nick-End Labeling
Pharmaceutical Preparations
Cytotoxins
Caspases
Genetic Therapy
Cause of Death
Cell Death
Genes
Therapeutics

Keywords

  • Apoptosis
  • Caski
  • Caspase-3
  • Cervical cancer
  • Chemotherapy
  • Cytotoxicity
  • Estrogen receptor
  • Gene therapy
  • MTT

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Heo, M. Y., Salama, S. A., Khatoon, N., Al-Hendy, A., & Au, W. W. (2008). Abrogation of estrogen receptor signaling augments cytotoxicity of anticancer drugs on caski cervical cancer cells. Anticancer research, 28(4 B), 2181-2187.

Abrogation of estrogen receptor signaling augments cytotoxicity of anticancer drugs on caski cervical cancer cells. / Heo, Moon Y.; Salama, Salama A.; Khatoon, Nilufar; Al-Hendy, Ayman; Au, William W.

In: Anticancer research, Vol. 28, No. 4 B, 01.07.2008, p. 2181-2187.

Research output: Contribution to journalArticle

Heo, MY, Salama, SA, Khatoon, N, Al-Hendy, A & Au, WW 2008, 'Abrogation of estrogen receptor signaling augments cytotoxicity of anticancer drugs on caski cervical cancer cells', Anticancer research, vol. 28, no. 4 B, pp. 2181-2187.
Heo, Moon Y. ; Salama, Salama A. ; Khatoon, Nilufar ; Al-Hendy, Ayman ; Au, William W. / Abrogation of estrogen receptor signaling augments cytotoxicity of anticancer drugs on caski cervical cancer cells. In: Anticancer research. 2008 ; Vol. 28, No. 4 B. pp. 2181-2187.
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