Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats

Toshio Ariga, Noriyuki Kasai, Ichiro Miyoshi, Masanaga Yamawaki, J. Neel Scarsdale, Robert K Yu, Takeshi Kasama, Takao Taki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 μg lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8% of total neutral glycolipids) and IV3Gal, IV2Fuc, GgOse4Cer (asialo-BGM1, 13.5%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2%), GlcCer (7.2%), LacCer (6.1%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.

Original languageEnglish (US)
Pages (from-to)257-266
Number of pages10
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1254
Issue number3
DOIs
StatePublished - Feb 9 1995
Externally publishedYes

Fingerprint

Ceramides
Glycolipids
Blood Group Antigens
Rats
Hepatocellular Carcinoma
Secondary Ion Mass Spectrometry
Liquid chromatography
Secondary ion mass spectrometry
Gas chromatography
Gas Chromatography
Lipids
Thin layer chromatography
Column chromatography
Liquids
Tumor Biomarkers
Thin Layer Chromatography
Liver
Chromatography
Spectrum Analysis
Adhesion

Keywords

  • Blood group B-active glycolipid
  • Hepatoma
  • Isogloboside
  • LEC rat
  • Neutral glycolipid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats. / Ariga, Toshio; Kasai, Noriyuki; Miyoshi, Ichiro; Yamawaki, Masanaga; Scarsdale, J. Neel; Yu, Robert K; Kasama, Takeshi; Taki, Takao.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1254, No. 3, 09.02.1995, p. 257-266.

Research output: Contribution to journalArticle

Ariga, Toshio ; Kasai, Noriyuki ; Miyoshi, Ichiro ; Yamawaki, Masanaga ; Scarsdale, J. Neel ; Yu, Robert K ; Kasama, Takeshi ; Taki, Takao. / Accumulation of isogloboside and ganglio-N-tetraosyl ceramide having blood group B determinant in the hepatomas of female LEC rats. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1995 ; Vol. 1254, No. 3. pp. 257-266.
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AB - We have studied the neutral glycolipid composition of spontaneous hepatomas in LEC female rats. Neutral lipid fractions were isolated and purified by column chromatographies on DEAE-Toyopearl 650(M) and Iatrobeads. The neutral glycolipid fraction contained 3.2 to 4.4 μg lipid-bound glucose (Glc) per mg protein, and consisted of isogloboside (iso-Gb4, 50.8% of total neutral glycolipids) and IV3Gal, IV2Fuc, GgOse4Cer (asialo-BGM1, 13.5%) as the major neutral glycolipids and Gb3 and iso-Gb3 (9.2%), GlcCer (7.2%), LacCer (6.1%) as the other species. The structure of iso-Gb4 was elucidated by gas-liquid chromatography (GLC), permethylation study, liquid secondary ion (LSI) mass spectrometry, and nuclear Overhauser enhancement spectroscopy (NOESY) and that for asialo-BGM1 by GLC, LSI mass spectrometry, and high-performance thin-layer chromatography (HPTLC)-overlay method using anti-asialo-BGM1 antibody. Isogloboside and asialo-BGM1 which are found in negligible amounts in normal liver tissues may represent excellent markers for studying tumor metastasis and cellular adhesion.

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