TY - JOUR
T1 - Accurate measurements of fetal hemoglobin for neonates with different gestational ages
AU - Shiao, Shyang Yun Pamela K.
AU - Ou, Ching Nan
N1 - Funding Information:
This study was supported in part by grant R01-NR04447 from the National Institutes of Health, Bethesda, MD, USA. The authors would also like to acknowledge the nurses, physicians, and respiratory therapists at the clinical settings who participated and helped with the blood sample collection, and Dr. Kohei Akazawa (Niigata University, Niigata, Japan) for statistical consultation and helpful reviews.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - The purposes of this study were to examine the accuracy of fetal hemoglobin (Hb F, α2γ2) as quickly measured by a hemoximeter but verified by high performance liquid chromatography [HPLC, including Hb F total (Hb Ft), acetylated Hb F (Hb F1), and non acetylated Hb F (Hb F*)], and to predict the Hb F levels for different gestational weeks of neonates. Thirty-nine neonates of predominantly Hispanic and African American ethnicity, with gestational ages ranging from 25 to 38 weeks, were investigated. Analyses were performed on 163 blood samples that were pure neonates' blood before the transfusion of any adult blood. Two neonates had increased Hb C [β6(A3)Glu→Lys, GAC→AAG] levels (1.67-2.79%) and one neonate whose mother drank alcohol during pregnancy, had elevated Hb A2 levels (0.12-0.14%). After excluding these data points, the mean Hb F were overestimated by hemoximeter, 118.4 ± 8.77% vs. 92.6 ± 2.77% by HPLC (mean difference: 25.8 ± 7.71%, p = <0.001). Mean Hb F1 was 10.5 ± 2.28%. Hb F levels decreased as gestational age increased (p <0.001 for Hb Ft and Hb F*; p = <0.05 for Hb F 1). A multivariate regression model for Hb F prediction was established with the best R2. The gestational age and post birth hours in the prediction of Hb Ft was included when Hb F could be determined at the clinical settings. Future studies may be needed to account for Hb F 1 when measuring Hb F levels to assess oxygenation status in (pre term) neonates.
AB - The purposes of this study were to examine the accuracy of fetal hemoglobin (Hb F, α2γ2) as quickly measured by a hemoximeter but verified by high performance liquid chromatography [HPLC, including Hb F total (Hb Ft), acetylated Hb F (Hb F1), and non acetylated Hb F (Hb F*)], and to predict the Hb F levels for different gestational weeks of neonates. Thirty-nine neonates of predominantly Hispanic and African American ethnicity, with gestational ages ranging from 25 to 38 weeks, were investigated. Analyses were performed on 163 blood samples that were pure neonates' blood before the transfusion of any adult blood. Two neonates had increased Hb C [β6(A3)Glu→Lys, GAC→AAG] levels (1.67-2.79%) and one neonate whose mother drank alcohol during pregnancy, had elevated Hb A2 levels (0.12-0.14%). After excluding these data points, the mean Hb F were overestimated by hemoximeter, 118.4 ± 8.77% vs. 92.6 ± 2.77% by HPLC (mean difference: 25.8 ± 7.71%, p = <0.001). Mean Hb F1 was 10.5 ± 2.28%. Hb F levels decreased as gestational age increased (p <0.001 for Hb Ft and Hb F*; p = <0.05 for Hb F 1). A multivariate regression model for Hb F prediction was established with the best R2. The gestational age and post birth hours in the prediction of Hb Ft was included when Hb F could be determined at the clinical settings. Future studies may be needed to account for Hb F 1 when measuring Hb F levels to assess oxygenation status in (pre term) neonates.
KW - Acetylated fetal Hb (Hb F)
KW - Adult Hb (Hb A)
KW - Fetal hemoglobin (Hb F)
KW - Non acetylated Hb F (Hb F)
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U2 - 10.1080/03630260600867883
DO - 10.1080/03630260600867883
M3 - Article
C2 - 16987797
AN - SCOPUS:33748940770
SN - 0363-0269
VL - 30
SP - 419
EP - 435
JO - Hemoglobin
JF - Hemoglobin
IS - 4
ER -