Abstract
To investigate urease-independent mechanisms by which Helicobacter pylori resists acid stress, subtractive RNA hybridization was used to identify H. pylori genes whose expression is induced after exposure to acid pH. This approach led to the isolation of a gene that encoded a predicted 34.8 kDa protein (WbcJ), which was homologous to known bacterial O-antigen biosynthesis proteins involved in the conversion of GDP-mannose to GDP- fucose. An isogenic wbcJ null mutant strain failed to express O-antigen and Lewis X or Lewis Y determinants and was more sensitive to acid stress than was the wild-type strain. Qualitative differences in LPS profiles were observed in H. pylori cells grown at pH 5 compared with pH 7, which suggests that H. pylori may alter its LPS structure in response to acidic pH. This may be an important adaptation facilitating H. pylori colonization of the acidic gastric environment.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 19-31 |
| Number of pages | 13 |
| Journal | Molecular Microbiology |
| Volume | 30 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 12 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Microbiology
Cite this
Acid-induced expression of an LPS-associated gene in Helicobacter pylori. / McGowan, Catherine C.; Necheva, Antoaneta; Thompson, Stuart A; Cover, Timothy L.; Blaser, Martin J.
In: Molecular Microbiology, Vol. 30, No. 1, 12.10.1998, p. 19-31.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acid-induced expression of an LPS-associated gene in Helicobacter pylori
AU - McGowan, Catherine C.
AU - Necheva, Antoaneta
AU - Thompson, Stuart A
AU - Cover, Timothy L.
AU - Blaser, Martin J.
PY - 1998/10/12
Y1 - 1998/10/12
N2 - To investigate urease-independent mechanisms by which Helicobacter pylori resists acid stress, subtractive RNA hybridization was used to identify H. pylori genes whose expression is induced after exposure to acid pH. This approach led to the isolation of a gene that encoded a predicted 34.8 kDa protein (WbcJ), which was homologous to known bacterial O-antigen biosynthesis proteins involved in the conversion of GDP-mannose to GDP- fucose. An isogenic wbcJ null mutant strain failed to express O-antigen and Lewis X or Lewis Y determinants and was more sensitive to acid stress than was the wild-type strain. Qualitative differences in LPS profiles were observed in H. pylori cells grown at pH 5 compared with pH 7, which suggests that H. pylori may alter its LPS structure in response to acidic pH. This may be an important adaptation facilitating H. pylori colonization of the acidic gastric environment.
AB - To investigate urease-independent mechanisms by which Helicobacter pylori resists acid stress, subtractive RNA hybridization was used to identify H. pylori genes whose expression is induced after exposure to acid pH. This approach led to the isolation of a gene that encoded a predicted 34.8 kDa protein (WbcJ), which was homologous to known bacterial O-antigen biosynthesis proteins involved in the conversion of GDP-mannose to GDP- fucose. An isogenic wbcJ null mutant strain failed to express O-antigen and Lewis X or Lewis Y determinants and was more sensitive to acid stress than was the wild-type strain. Qualitative differences in LPS profiles were observed in H. pylori cells grown at pH 5 compared with pH 7, which suggests that H. pylori may alter its LPS structure in response to acidic pH. This may be an important adaptation facilitating H. pylori colonization of the acidic gastric environment.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031696167&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2958.1998.t01-1-01079.x
DO - 10.1046/j.1365-2958.1998.t01-1-01079.x
M3 - Article
VL - 30
SP - 19
EP - 31
JO - Molecular Microbiology
JF - Molecular Microbiology
SN - 0950-382X
IS - 1
ER -